Revision 1
#36349
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
300
Source/Isotype:
Rabbit IgG
UniProt ID:
#P02751-7
Entrez-Gene Id:
2335
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
Fibronectin-EDB/FN1-EDB (E8J3Z) Rabbit mAb recognizes endogenous levels of the EDB isoform of Fibronectin/FN1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the EDB domain of human Fibronectin/FN1 protein.
Background
Fibronectin is a multi-domain extracellular matrix (ECM) protein. The protein uses its different domains to bind distinct ECM components such as collagens, growth factors, and cell surface integrins to carry on its functions (1). Fibronectin has been implicated in many essential biological processes, including tissue repair, fibrosis, and tumor development (1,2). There are many fibronectin isoforms. Plasma fibronectin is synthesized by hepatocytes and exists as a compact, inactive conformation in the bloodstream. It is a major component of fibrin clots. Upon binding to integrins or other cell surface receptors, fibronectin switches to an extended conformation, and exposes its function domains to activated extracellular matrix assembly (3). Fibroblasts, endothelial cells, and many types of cancer cells have also been shown to synthesize cellular fibronectin isoforms. Among them, the EDA or EDB isoforms are particularly highly expressed during fibrosis and cancer development, and are potential diagnostic and therapeutic targets (4-6).
Background References
- Zollinger, A.J. and Smith, M.L. (2017) Matrix Biol 60-61, 27-37.
- Wang, K. et al. (2016) Cell Mol Bioeng 9, 1-11.
- To, W.S. and Midwood, K.S. (2011) Fibrogenesis Tissue Repair 4, 21.
- Kaspar, M. et al. (2006) Int J Cancer 118, 1331-9.
- Wang, J.P. and Hielscher, A. (2017) J Cancer 8, 674-82.
- Zent, J. and Guo, L.W. (2018) Cell Physiol Biochem 49, 848-68.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing human Fibronectin-EDB/FN1-EDB fragment (hFibronectin-EDB/FN1-EDB fragment; +), using Fibronection-EDB/FN1-EDB (E8J3Z) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western blot analysis of extracts from OV-90 and Hep G2 cells using Fibronectin-EDB/FN1-EDB (E8J3Z) Rabbit mAb (upper), Fibronectin/FN1 (E5H6X) Rabbit mAb #26836 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).