Revision 5
#4402
Store at -20C
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, IHC-P, FC-FP, ChIP, C&R
Reactivity:
H M R Mk
Sensitivity:
Endogenous
MW (kDa):
82-90
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q9H334
Entrez-Gene Id:
27086
Product Usage Information
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:200 |
Immunohistochemistry (Paraffin) | 1:100 |
Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Chromatin IP | 1:100 |
CUT&RUN | 1:50 |
Storage
For a carrier free (BSA and azide free) version of this product see product #85650.
Specificity/Sensitivity
FoxP1 (D35D10) XP® Rabbit mAb detects endogenous levels of total FoxP1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu584 of human FoxP1 protein.
Background
Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5).
FoxP1 plays an important role in cardiac valve and B cell development. Targeted deletion of FoxP1 in mice results in embryonic death at E14.5 with severe defects in cardiac outflow development, endocardial cushion morphogenesis, and myocyte proliferation and maturation (6). The FoxP1 gene is located on chromosome 3p14.1 and loss of heterozygosity of this region in multiple cancer types suggests a role for FoxP1 as a tumor suppressor (7). Alternatively, FoxP1 may also act as an oncogene as it is highly expressed in a variety of B cell malignancies and is frequently involved in chromosome translocation (8,9).
Background References
- Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
- Shu, W. et al. (2001) J Biol Chem 276, 27488-97.
- Lu, M.M. et al. (2002) Gene Expr Patterns 2, 223-8.
- Koon, H.B. et al. (2007) Expert Opin Ther Targets 11, 955-65.
- Li, S. et al. (2004) Mol Cell Biol 24, 809-22.
- Wang, B. et al. (2004) Development 131, 4477-87.
- Banham, A.H. et al. (2001) Cancer Res 61, 8820-9.
- Wlodarska, I. et al. (2005) Leukemia 19, 1299-305.
- Goatly, A. et al. (2008) Mod Pathol 21, 902-11.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) FC-FP: Flow Cytometry (Fixed/Permeabilized) ChIP: Chromatin IP C&R: CUT&RUN
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 5
Western blot analysis of extracts from various cell lines using FoxP1 (D35D10) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using FoxP1 (D35D10) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Flow cytometric analysis of MOLT-4 cells using FoxP1 (D35D10) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Revision 5
Chromatin immunoprecipitations were performed with cross-linked chromatin from Raji cells and either FoxP1 (D35D10) XP® Rabbit mAb, or Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human BCL2 Promoter Primers #12924, SimpleChIP® Human HSP90B1 Promoter Primers #12899, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
CUT&RUN was performed with Raji cells and FoxP1 (D35D10) XP® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA library was prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figure shows binding across USP5 gene, a known target gene of FoxP1 (see additional figure containing CUT&RUN-qPCR data).
CUT&RUN was performed with Raji cells and FoxP1 (D35D10) XP® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA library was prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 12 (upper), including USP5 (lower), a known target gene of FoxP1 (see additional figure containing CUT&RUN-qPCR data).
Revision 5
CUT&RUN was performed with Raji cells and either FoxP1 (D35D10) XP® Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human USP5 promoter primers and human MYOM3 promoter primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.