WB, IP, FC-L
H
Endogenous
50-80, 110-140
Rabbit IgG
#Q99062
1441
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Flow Cytometry (Live) | 1:50 - 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human G-CSF-R protein.
Background
Granulocyte colony-stimulating factor receptor (G-CSF-R) is a transmembrane protein comprised of an immunoglobulin-like (Ig-like) domain, a cytokine receptor-homologous (CRH) domain, and three fibronectin type III (FN III) domains (1). G-CSF-R is expressed on all granulocytic lineages, including progenitor cells, and has been detected on monocytes, T and B lymphocytes, as well as non-hematopoietic tissues including cardiomyocytes and neural stem cells (2-6). The primary ligand for G-CSF-R is the cytokine granulocyte colony-stimulating factor (G-CSF). G-CSF-R has no intrinsic tyrosine kinase activity; ligand binding induces conformational changes in the receptor, leading to activation of the Jak/Stat, PI3K/Akt, and MAPK pathways (7-10). G-CSF induces differentiation and proliferation of myeloid progenitor cells into neutrophils (11). Multiple diseases have been associated with mutations of the G-CSF-R gene, CSF3R, including severe congenital neutropenia (SCN), chronic neutrophilic leukemia (CNL), and atypical chronic myeloid leukemia (aCML) (12).
- Fukunaga, R. et al. (1991) EMBO J 10, 2855-65.
- Nicola, N.A. and Metcalf, D. (1984) Proc Natl Acad Sci U S A 81, 3765-9.
- Boneberg, E.M. et al. (2000) Blood 95, 270-6.
- Morikawa, K. et al. (2002) Br J Haematol 118, 296-304.
- Harada, M. et al. (2005) Nat Med 11, 305-11.
- Schneider, A. et al. (2005) J Clin Invest 115, 2083-98.
- Fukunaga, R. et al. (1993) Cell 74, 1079-87.
- Nicholson, S.E. et al. (1994) Proc Natl Acad Sci U S A 91, 2985-8.
- Zhu, Q.S. et al. (2006) Blood 107, 1847-56.
- Bashey, A. et al. (1994) Blood 83, 949-57.
- Touw, I.P. et al. (2013) Hematol Oncol Clin North Am 27, 61-73, viii.
- Dwivedi, P. and Greis, K.D. (2017) Exp Hematol 46, 9-20.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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