WB, IP, FC-L
H
Endogenous
50-80, 110-140
Rabbit IgG
#Q99062
1441
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Flow Cytometry (Live) | 1:50 - 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human G-CSF-R protein.
Background
Granulocyte colony-stimulating factor receptor (G-CSF-R) is a transmembrane protein comprised of an immunoglobulin-like (Ig-like) domain, a cytokine receptor-homologous (CRH) domain, and three fibronectin type III (FN III) domains (1). G-CSF-R is expressed on all granulocytic lineages, including progenitor cells, and has been detected on monocytes, T and B lymphocytes, as well as non-hematopoietic tissues including cardiomyocytes and neural stem cells (2-6). The primary ligand for G-CSF-R is the cytokine granulocyte colony-stimulating factor (G-CSF). G-CSF-R has no intrinsic tyrosine kinase activity; ligand binding induces conformational changes in the receptor, leading to activation of the Jak/Stat, PI3K/Akt, and MAPK pathways (7-10). G-CSF induces differentiation and proliferation of myeloid progenitor cells into neutrophils (11). Multiple diseases have been associated with mutations of the G-CSF-R gene, CSF3R, including severe congenital neutropenia (SCN), chronic neutrophilic leukemia (CNL), and atypical chronic myeloid leukemia (aCML) (12).
- Fukunaga, R. et al. (1991) EMBO J 10, 2855-65.
- Nicola, N.A. and Metcalf, D. (1984) Proc Natl Acad Sci U S A 81, 3765-9.
- Boneberg, E.M. et al. (2000) Blood 95, 270-6.
- Morikawa, K. et al. (2002) Br J Haematol 118, 296-304.
- Harada, M. et al. (2005) Nat Med 11, 305-11.
- Schneider, A. et al. (2005) J Clin Invest 115, 2083-98.
- Fukunaga, R. et al. (1993) Cell 74, 1079-87.
- Nicholson, S.E. et al. (1994) Proc Natl Acad Sci U S A 91, 2985-8.
- Zhu, Q.S. et al. (2006) Blood 107, 1847-56.
- Bashey, A. et al. (1994) Blood 83, 949-57.
- Touw, I.P. et al. (2013) Hematol Oncol Clin North Am 27, 61-73, viii.
- Dwivedi, P. and Greis, K.D. (2017) Exp Hematol 46, 9-20.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.