Revision 1

#96462

Store at -20C

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Orders:

877-616-CELL (2355)

orders@cellsignal.com

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-P, IF-IC

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
53, 30

Source/Isotype:
Rabbit IgG

UniProt ID:
#P57764

Entrez-Gene Id:
79792

Product Usage Information

This product is the carrier free version of product #69469. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #69469

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity/Sensitivity

Gasdermin D (E5O4N) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total Gasdermin D protein. This antibody will recognize the 30 kDa amino-terminal fragment produced during pyroptosis.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to a fragment within the the amino-terminal pore-forming region of human Gasdermin D protein.

Background

Gasdermin D (GSDMD), a member of the gasdermin family that includes GSDMA, GSDMB, and GSDMC, has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

W: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from control PC-3 cells (lane 1) or CRISPR/Cas9 Gasdermin D knockout (KO) PC-3 cells (lane 2) using Gasdermin D (E5O4N) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 1: Gasdermin D (E5O4N) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using Gasdermin D (E5O4N) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of Gasdermin D protein in IMR-32 cells is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.
Western Blotting Image 2: Gasdermin D (E5O4N) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from THP-1 cells, differentiated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (50 ng/mL, 24 hr and then rested 48 hr) followed by vehicle (-) or treated with Lipopolysaccharides (LPS) #14011 (5 μg/mL, 6 hr; +), using Gasdermin D (E5O4N) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 3: Gasdermin D (E5O4N) Rabbit mAb (BSA and Azide Free)
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human infiltrating ductal carcinoma of the breast using Gasdermin D (E5O4N) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Gasdermin D (E5O4N) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Gasdermin D (E5O4N) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Gasdermin D (E5O4N) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using Gasdermin D (E5O4N) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Gasdermin D (E5O4N) Rabbit mAb (BSA and Azide Free)
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human small intestine using Gasdermin D (E5O4N) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: Gasdermin D (E5O4N) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human colon using Gasdermin D (E5O4N) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: Gasdermin D (E5O4N) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human spleen using Gasdermin D (E5O4N) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: Gasdermin D (E5O4N) Rabbit mAb (BSA and Azide Free)
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Gasdermin D (E5O4N) Rabbit mAb (left) or a Gasdermin D Antibody (right). These two antibodies detect unique, non-overlapping epitopes on human Gasdermin D. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: Gasdermin D (E5O4N) Rabbit mAb (BSA and Azide Free)
Lymph Node N.4: Immunohistochemical analysis of paraffin-embedded normal human lymph node using Gasdermin D (E5O4N) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: Gasdermin D (E5O4N) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded HCC1937 cell pellet (left, positive) or MCF7 cell pellet (right, negative) using Gasdermin D (E5O4N) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 9: Gasdermin D (E5O4N) Rabbit mAb (BSA and Azide Free)
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Confocal immunofluorescent analysis of control PC-3 cells (left, positive) or CRISPR/Cas9 Gasdermin D knockout (KO) PC-3 cells (right, negative) using Gasdermin D (E5O4N) Rabbit mAb (green), β-Actin (8H10D10) Mouse mAb #3700 (red), or DAPI #4083 (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: Gasdermin D (E5O4N) Rabbit mAb (BSA and Azide Free)
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.