Revision 1
#40618
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
M R
Sensitivity:
Endogenous
MW (kDa):
57, 33
Source/Isotype:
Rabbit
UniProt ID:
#Q9Z2D3
Entrez-Gene Id:
54722
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
Gasdermin E Antibody recognizes endogenous levels of total Gasdermin E protein. This antibody can also detect the amino-terminal cleavage fragment associated with pyroptosis.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His112 of mouse Gasdermin E protein. Antibodies are purified by peptide affinity chromatography.
Background
The gasdermin family, which includes GSDMA, GSDMB, GSDMC, GSDMD, and GSDME, has been shown to play a role in inflammation and cell death. Gasdermin D has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7).
Gasdermin E (GSDME), also known as DFNA5, was originally identified as a genetic cause of nonsyndromic hearing loss (8). Like other gasdermin family members, Gasdermin E contains an amino-terminal pore forming domain that triggers pyroptosis. Cleavage of Gasdermin E at Asp270 is induced by apoptotic-associated Caspase-3, converting apoptotic signals to pyroptosis (9). In addition, cleavage of Gasdermin E can be induced by Granzyme B secreted by NK cells and contributes to tumor suppressive activity (10). Gasdermin E expression is suppressed in several types of cancer including gastric, colorectal, and breast carcinoma, and may be associated with decreased survival (11-13). In contrast, an increase in Gasdermin E, including the amino-terminal pore-forming fragment, is associated with conditions of excessive inflammation (14-16).
Background References
- Kayagaki, N. et al. (2015) Nature 526, 666-71.
- Shi, J. et al. (2015) Nature 526, 660-5.
- Broz, P. and Dixit, V.M. (2016) Nat Rev Immunol 16, 407-20.
- Aglietti, R.A. et al. (2016) Proc Natl Acad Sci USA 113, 7858-63.
- Ding, J. et al. (2016) Nature 535, 111-6.
- Liu, X. et al. (2016) Nature 535, 153-8.
- Sborgi, L. et al. (2016) EMBO J 35, 1766-78.
- Van Laer, L. et al. (1998) Nat Genet 20, 194-7.
- Rogers, C. et al. (2017) Nat Commun 8, 14128.
- Zhang, Z. et al. (2020) Nature 579, 415-420.
- Kim, M.S. et al. (2008) Oncogene 27, 3624-34.
- Kim, M.S. et al. (2008) Biochem Biophys Res Commun 370, 38-43.
- Yokomizo, K. et al. (2012) Anticancer Res 32, 1319-22.
- Tan, G. et al. (2021) Cell Rep 35, 109265.
- Li, Y. et al. (2021) Cell Death Differ 28, 2333-2350.
- Shi, H. et al. (2021) Circ Res 129, 383-396.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
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Revision 1
#40618
Gasdermin E Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse Gasdermin E protein (mGSDME-Myc/DDK; +), using Gasdermin E Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western blot analysis of extracts from P815 cells, untreated (-) or treated with raptinal (10 μM, 2 hr; +), using Gasdermin E Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb (lower).
Western blot analysis of extracts from various cell lines using Gasdermin E Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).