Revision 1
#24253
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IF-IC, FC-FP
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
13-15
Source/Isotype:
Rabbit IgG
UniProt ID:
#P22749
Entrez-Gene Id:
10578
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
Storage
Specificity/Sensitivity
GNLY (E3N1M) Rabbit mAb recognizes endogenous levels of both long and short isoforms of human GNLY protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human GNLY protein.
Background
Granulysin (GNLY) was originally identified as a late activation marker in T cells, and it is expressed by killer lymphocytes in most mammals, but not rodents. GNLY is largely confined to cytotoxic granules but can be secreted by killer cells, especially those expressing high levels of GNLY, such as decidual natural killer (NK) cells (1-3). GNLY is produced as a 15 kDa protein and is processed into a 9 kDa active pore-forming fragment by proteolytic removal of peptides from both the N- and C-termini. Moreover, the 15 kDa form was reported to function as an immune alarmin, causing the maturation and migration of antigen-presenting cells and other immune cells (4-7). Unlike perforin, a cholesterol-dependent pore-forming protein that preferentially permeabilizes mammalian membranes, GNLY is inhibited by cholesterol and forms pores much more efficiently in microbial than mammalian membranes, and it plays an important role against bacteria, fungi, and parasite infections (8,9).
Background References
- Jongstra, J. et al. (1987) J Exp Med 165, 601-14.
- Pitabut, N. et al. (2011) Microbiol Immunol 55, 565-73.
- Crespo, Â.C. et al. (2020) Cell 182, 1125-1139.e18.
- Hanson, D.A. et al. (1999) Mol Immunol 36, 413-22.
- Tewary, P. et al. (2010) Blood 116, 3465-74.
- Clayberger, C. et al. (2012) J Immunol 188, 6119-26.
- Sparrow, E. and Bodman-Smith, M.D. (2020) Immunol Lett 217, 126-132.
- Kumar, J. et al. (2001) Expert Opin Investig Drugs 10, 321-9.
- Dotiwala, F. and Lieberman, J. (2019) Curr Opin Immunol 60, 19-29.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
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