Render Target: STATIC
Render Timestamp: 2025-01-02T10:45:14.331Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-09-30 01:57:19.992
Product last modified at: 2025-01-01T09:06:42.757Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GREB1 (E8M3F) Rabbit mAb #65171

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 216
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GREB1 (E8M3F) Rabbit mAb recognizes endogenous levels of total GREB1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro242 of human GREB1 protein.

    Background

    GREB1 was first identified as a gene with a strong response to estrogen receptor (ER) activity (1,2). It has been shown to bind chromatin as an ER-coactivator where it stabilizes interactions with other ER-related cofactors (3). In tamoxifen resistance, Ezh2 represses expression of GREB1, which retargeted ER coregulators (4). In addition to having numerous implications in ER-based pathologies, GREB1 expression has also been found in prostate cancer, where it is regulated by androgen (5). Similarly to breast cancer, GREB1 seems to function as a tumor suppressor and its expression has been found to be correlated with a more favorable prognosis (6). However, GREB1 has also been shown to amplify androgen receptor (AR) output, resulting in enzalutamide resistance (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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