Revision 1
#12604
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, IF-IC, FC-FP
Reactivity:
H Mk
Sensitivity:
Endogenous
MW (kDa):
60
Source/Isotype:
Rabbit IgG
UniProt ID:
#O94992
Entrez-Gene Id:
10614
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Immunofluorescence (Immunocytochemistry) | 1:1200 |
Flow Cytometry (Fixed/Permeabilized) | 1:1600 |
Storage
Specificity/Sensitivity
HEXIM1 (D5Y5K) Rabbit mAb recognizes endogenous levels of total HEXIM1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly63 of human HEXIM1 protein.
Background
Hexamethylene bis-acetamide-inducible protein 1 (HEXIM1) was originally identified in vascular smooth muscle cells as a protein that is upregulated upon treatment with the differentiating agent hexamethylene bisacetamide (1). HEXIM1 binds 7SK RNA, a highly abundant non-coding RNA, and together they act as a potent inhibitor of positive transcription elongation factor b (P-TEFb) (2,3). P-TEFb phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II and is an important regulator of transcription elongation (4-8). 7SK RNA-bound HEXIM1 interacts with the cyclin T1 subunit of P-TEFb, sequestering P-TEFb in an inactive form leading to transcription inhibition (2,3). The regulation of the relative ratio of inactive to active P-TEFb in the cell by HEXIM1/7SK RNA is thought to play a critical role in regulation of a wide range of cellular gene expression programs such as estrogen and glucocorticoid receptor regulated genes (9-12).
Background References
- Ouchida, R. et al. (2003) Genes Cells 8, 95-107.
- Michels, A.A. et al. (2004) EMBO J 23, 2608-19.
- Yik, J.H. et al. (2003) Mol Cell 12, 971-82.
- Buratowski, S. (2009) Mol Cell 36, 541-6.
- Lenasi, T. and Barboric, M. RNA Biol 7, 145-50.
- Pirngruber, J. et al. (2009) Cell Cycle 8, 3636-42.
- Wada, T. et al. (1998) EMBO J 17, 7395-403.
- Yamada, T. et al. (2006) Mol Cell 21, 227-37.
- Peterlin, B.M. et al. (2012) Wiley Interdiscip Rev RNA 3, 92-103.
- Ketchart, W. et al. (2011) Oncogene 30, 3563-9.
- Ogba, N. et al. (2008) Cancer Res 68, 7015-24.
- Shimizu, N. et al. (2005) Proc Natl Acad Sci U S A 102, 8555-60.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
#12604
HEXIM1 (D5Y5K) Rabbit mAb
Western blot analysis of extracts from various cell lines using HEXIM1 (D5Y5K) Rabbit mAb
Immunoprecipitation of HEXIM1 from HeLa cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or HEXIM1 (D5Y5K) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using HEXIM1 (D5Y5K) Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells using HEXIM1 (D5Y5K) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Revision 1
#12604
HEXIM1 (D5Y5K) Rabbit mAb
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with JQ1 (500nM, 72 hours; green), using HEXIM1 (D5Y5K) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) was used as a secondary antibody.