Revision 1
#25328
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877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IHC-Bond, IHC-P
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
30-40
Source/Isotype:
Rabbit IgG
UniProt ID:
#P01903
Entrez-Gene Id:
3122
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Supplied in 1X PBS, BSA and Azide Free.
For standard formulation of this product see product #97971.
Storage
Specificity/Sensitivity
HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total HLA-DRA protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val153 of human HLA-DRA protein.
Background
Major histocompatibility complex class II (MHC class II) molecules are heterodimeric, transmembrane glycoproteins expressed on the surface of antigen-presenting cells, such as macrophages, dendritic cells, and B cells. Expression can also be induced on other cell types through interferon-γ signaling (1). Prior to being displayed on the cell membrane, MHC class II molecules are loaded with exogenous peptide antigens approximately 15-24 amino acids in length that were derived from endocytosed extracellular proteins digested in the lysosome (2). Antigen-presentation through MHC class II is required for T cell activation during the immune response to extracellular pathogens (2). In humans, the MHC class II protein complex is encoded by the human leukocyte antigen gene complex (HLA). HLAs corresponding to MHC class II are HLA-DP, HLA-DM, HLA-DOA, HLA-DOB, HLA-DQ, and HLA-DR (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
W: Western Blotting IHC-Bond: IHC Leica Bond
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with constructs expressing full-length human Myc/DDK-tagged HLA-DRA (hHLA-DRA-Myc/DDK), HLA-DPA1 (hHLA-DPA1-Myc/DDK), HLA-DOA (hHLA-DOA-Myc/DDK), HLA-DQA1 (hHLA-DQA1-MycDDK), HLA-DQA2 (hHLA-DQA2-Myc/DKK), or HLA-DMA (hHLA-DMA-Myc/DKK), using HLA-DRA (E9R2Q) XP® Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Western blot analysis of extracts from various cell lines using HLA-DRA (E9R2Q) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human bladder adenocarcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb performed on the Leica® BOND™ Rx. Data were generated using the standard formulation of this product.
Revision 1
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb performed on the Leica® BOND™ Rx. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb performed on the Leica® BOND™ Rx. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded Raji cell pellet (left, positive) or Jurkat cell pellet (right, negative) using HLA-DRA (E9R2Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Revision 1
Immunohistochemical analysis of paraffin-embedded human mucoepidermoid carcinoma of the larynx using HLA-DRA (E9R2Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using HLA-DRA (E9R2Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Revision 1
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded ovarian carcinoma (left) or colon carcinoma (right) using HLA-DRA (E9R2Q) XP® Rabbit mAb (top) or HLA-DRA Rabbit mAb (bottom). These two antibodies detect independent, unique epitopes on human HLA-DRA. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.