WB, IP
H
Endogenous
95, 105
Rabbit IgG
#Q6UWB1
9466
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human IL-27RA protein.
Background
Interleukin 27 receptor α (IL-27RA or IL-27Rα), also known as T cell cytokine receptor (TCCR) and WSX-1, forms a receptor complex with gp130 to bind and enable IL-27 signaling (1,2). IL-27Rα is expressed on T cells, B cells, natural killer (NK) cells, monocytes, macrophages, dendritic cells (DCs), as well as on subsets of epithelial and endothelial cells (3). Binding of IL-27 to its receptor complex induces the activation of receptor-associated Jak1 and Jak2, which in turn phosphorylates Stat1 and Stat3 (4,5). IL-27 signaling plays an important anti-inflammatory role in chronic immune-mediated disease and infectious disease, and enhances the expression of co-inhibitory molecules LAG-3, TIM-3, PD-1, and TIGIT (6-8). Due to its immunosuppressive role in cancers, IL-27 has been identified as a target for cancer immunotherapy (9).
- Chen, Q. et al. (2000) Nature 407, 916-20.
- Sprecher, C.A. et al. (1998) Biochem Biophys Res Commun 246, 82-90.
- Caveney, N.A. et al. (2022) Elife 11, e78463. doi: 10.7554/eLife.78463.
- Lucas, S. et al. (2003) Proc Natl Acad Sci USA 100, 15047-52.
- Owaki, T. et al. (2008) J Immunol 180, 2903-11.
- Stumhofer, J.S. et al. (2006) Nat Immunol 7, 937-45.
- Koltsova, E.K. et al. (2012) Circ Res 111, 1274-85.
- Chihara, N. et al. (2018) Nature 558, 454-459.
- Aghayev, T. et al. (2022) Cancer Discov 12, 1960-1983.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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