Revision 1
#98649
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877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IHC-Bond, IHC-P, IF-F, IF-IC, FC-FP
Reactivity:
H M R
Sensitivity:
Endogenous
MW (kDa):
95, 220
Source/Isotype:
Rabbit IgG
UniProt ID:
#P06213
Entrez-Gene Id:
3643
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Supplied in 1X PBS, BSA and Azide Free.
For standard formulation of this product see product #23413
Storage
Specificity/Sensitivity
Insulin Receptor β (E9L5V) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total insulin receptor β. The 50 kDa band(s) seen on western blot is a probable partial degradation product of insulin receptor β.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1374 of human insulin receptor.
Background
Insulin receptor (InsR) is a heterodimeric membrane receptor tyrosine kinase. It is composed of an extracellular α-subunit containing the ligand binding domain, a β-subunit containing an extracellular domain, a transmembrane domain, and a cytoplasmic tyrosine kinase domain (1). Binding of insulin to InsR results in receptor autophosphorylation and subsequent tyrosine kinase activation (2). This provides a docking site for various adaptor molecules, including insulin receptor substrate (IRS), Gab, and Shc, phosphorylation of which promotes subsequent activation of multiple downstream signaling pathways, including MAPK, PI3K, and TC10 (3,4). These events lead to increased glucose uptake and metabolism, and can promote cell growth. Loss-of-function mutation or desensitization of the InsR are two major contributors to insulin resistance and Type 2 diabetes (5).
Background References
- Yip, C.C. and Ottensmeyer, P. (2003) J Biol Chem 278, 27329-32.
- Hubbard, S.R. (2013) Cold Spring Harb Perspect Biol 5, a008946.
- Saltiel, A.R. and Pessin, J.E. (2002) Trends Cell Biol 12, 65-71.
- Zick, Y. (2001) Trends Cell Biol 11, 437-41.
- Boucher, J. et al. (2014) Cold Spring Harb Perspect Biol 6, pii: a009191. doi: 10.1101/cshperspect.a009191.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
W: Western Blotting IHC-Bond: IHC Leica Bond IF-F: Immunofluorescence (Frozen) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
Western blot analysis of extracts from various cell lines and tissues using Insulin Receptor β (E9L5V) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Western blot analysis of extracts from RL and U266 cells using Insulin Receptor β (E9L5V) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Insulin Receptor β (E9L5V) XP® Rabbit mAb performed on the Leica® BOND™ Rx. Data were generated using the standard formulation of this product.
Revision 1
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma using Insulin Receptor β (E9L5V) XP® Rabbit mAb performed on the Leica® BOND™ Rx. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Insulin Receptor β (E9L5V) XP® Rabbit mAb performed on the Leica® BOND™ Rx. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded Hep 3B2.1-7 cell pellet (left, positive) or HT-1080 cell pellet (right, negative) using Insulin Receptor β (E9L5V) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Revision 1
Immunohistochemical analysis of paraffin-embedded human mucoepidermoid carcinoma of the larynx using Insulin Receptor β (E9L5V) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using Insulin Receptor β (E9L5V) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using Insulin Receptor β (E9L5V) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Revision 1
Immunohistochemical analysis of paraffin-embedded mouse small intestine using Insulin Receptor β (E9L5V) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of fixed frozen mouse liver labeled with Insulin Receptor β (E9L5V) XP® Rabbit mAb (left, green) and co-labeled with DyLight™ 650 Phalloidin #12956 (right, red), and DAPI #4083 (right, blue). Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of HCC1419 cells (left, positive) or HT-1080 cells (right, negative) using Insulin Receptor β (E9L5V) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
Revision 1
Flow cytometric analysis of RL cells (blue) and U266 cells (green) using Insulin Receptor β (E9L5V) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.