Revision 1
#37135
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
105
Source/Isotype:
Rabbit IgG
UniProt ID:
#P48200
Entrez-Gene Id:
3658
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
IRP2 (D6E6W) Rabbit mAb recognizes endogenous levels of total IRP2 protein. It does not recognize human IRP1.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro731 of human IRP2 protein.
Background
Iron regulatory proteins (IRPs; also known as IREBs) are RNA-binding proteins that recognize iron-responsive elements (IREs) and play an important role in maintaining iron homeostasis in mammalian cells. IREs are conserved cis-regulatory hairpin structures located within the 5’ or 3’ untranslated regions (UTRs) of target mRNAs. IRPs inhibit translation when bound to IREs within the 5’ UTR of mRNA encoding for proteins involved in iron storage, export, and utilization. IRP binding to multiple IREs within the 3’ UTR of transferin receptor 1 (TFR1) mRNA prevents its degradation, thereby augmenting translation of TFR1 and increasing iron uptake into cells (1-3). Dysregulation of IRPs has been associated with human cancers (4-6).
In iron replete cells, FBXL5 targets IRP2 for degradation. Under iron deficiency and/or hypoxic conditions, FBXL5 is destabilized, resulting in IRP2 accumulation and interaction with IRE-containing mRNA (7).
Background References
- Rouault, T.A. (2006) Nat Chem Biol 2, 406-14.
- Wang, J. and Pantopoulos, K. (2011) Biochem J 434, 365-81.
- Pantopoulos, K. et al. (2012) Biochemistry 51, 5705-24.
- Haro, K.J. et al. (2012) PLoS One 7, e48841.
- Wang, W. et al. (2014) Cancer Res 74, 497-507.
- Jeong, S.M. et al. (2015) Oncogene 34, 2115-24.
- Moroishi, T. et al. (2011) Cell Metab 14, 339-51.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
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Limited Uses
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Revision 1
#37135
IRP2 (D6E6W) Rabbit mAb
Western blot analysis of extracts from 293 cells, treated overnight with ammonium iron(III) citrate (FAC; 50 μM) or with deferoxamine mesylate (DFO; 50 μM), using IRP2 (D6E6W) Rabbit mAb.