Revision 1
#64099
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP
Reactivity:
H M R
Sensitivity:
Endogenous
MW (kDa):
85
Source/Isotype:
Rabbit
UniProt ID:
#Q07954
Entrez-Gene Id:
4035
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity/Sensitivity
LRP1 Antibody recognizes endogenous levels of total LRP1 β subunit.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LRP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Low density lipoprotein receptor related protein 1 (LRP1) is a type I transmembrane receptor that mediates the endocytosis of various ligands (1). LRP1 plays important roles in lipid homeostasis, signaling transduction, embryonic development, and glucose metabolism (2-6). In addition, LRP1 regulates APP processing and facilitates the clearance of beta-amyloid (7-9). This finding makes LRP1 a potential therapeutic target for Alzheimer’s disease. LRP1 preprotein is proteolytically processed by furin to generate a 515 kDa extracellular α subunit and a membrane-anchored 85 kDa β subunit, which together form the mature receptor (10).
Background References
- Lillis, A.P. et al. (2008) Physiol Rev 88, 887-918.
- Herz, J. et al. (1988) EMBO J 7, 4119-27.
- van der Geer, P. (2002) Trends Cardiovasc Med 12, 160-5.
- May, P. and Herz, J. (2003) Traffic 4, 291-301.
- Zilberberg, A. et al. (2004) J Biol Chem 279, 17535-42.
- Hofmann, S.M. et al. (2007) J Clin Invest 117, 3271-82.
- Cam, J.A. et al. (2005) J Biol Chem 280, 15464-70.
- Bu, G. et al. (2006) Ann N Y Acad Sci 1086, 35-53.
- Bell, R.D. et al. (2009) Nat Cell Biol 11, 143-53.
- Willnow, T.E. et al. (1996) Biochem J 313 (Pt 1), 71-6.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
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Revision 1
#64099
LRP1 Antibody
Western blot analysis of extracts from U-87 MG and A549 cells using LRP1 Antibody.