Revision 1
#9039
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, IHC-P, IF-F
Reactivity:
H M R
Sensitivity:
Endogenous
MW (kDa):
37-45
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q9ULP0
Entrez-Gene Id:
65009
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Immunohistochemistry (Paraffin) | 1:800 |
Immunofluorescence (Frozen) | 1:200 |
Storage
Specificity/Sensitivity
NDRG4 (D4A6) Rabbit mAb recognizes endogenous levels of total NDRG4 protein.
Species predicted to react based on 100% sequence homology
Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly217 of human NDRG4 protein.
Background
The NDRG (N-Myc downstream-regulated gene) family consisting of NDRG1, NDRG2, NDRG3, and NDRG4 are structurally related proteins with roles in cell proliferation, differentiation, apoptosis, stress responses, and cell migration/metastasis (1-3). NDRG1 was originally identified as a protein that was upregulated in N-Myc knockout mice (1). Proteins in the NDRG family, particularly NDRG1 and NDRG2, have been reported to be down-regulated in various cancer tissues and have been suggested to function as a tumor suppressors (4,5).
Unlike other members of the family that are widely expressed, NDRG4/Bdm1 expression is restricted primarily to the heart and brain (2,3,6,7). Multiple alternatively spliced isoforms of NDRG4 have been reported (3,7). Expression of NDRG4 is reduced in the brain of patients with Alzheimer's disease (3). In contrast, NDRG4 expression is elevated in glioblastoma and contributes to cell cycle progression and survival (8). NDRG4 also may be inactivated by promotor methylation in colorectal cancer and function as a tumor suppressor gene (9).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
#9039
NDRG4 (D4A6) Rabbit mAb
Western blot analysis of extracts from human cerebellum, mouse brain, and rat brain using NDRG4 (D4A6) Rabbit mAb.
Western blot analysis of extracts from various cell lines using NDRG4 (D4A6) Rabbit mAb.
Western blot analysis of 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human NDRG4 (hNDRG4-Myc/DDK, +), using NDRG4 (D4A6) Rabbit mAb.
Revision 1
#9039
NDRG4 (D4A6) Rabbit mAb
Immunohistichemical analysis of paraffin-embedded human lung carcinoma using NDRG4 (D4A6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse brain using NDRG4 (D4A6) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide(right).
Confocal immunofluorescent analysis of adult mouse brain using NDRG4 (D4A6) Rabbit mAb and Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8039 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).