Revision 3
#72379
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, W-S, IP
Reactivity:
H M R
Sensitivity:
Endogenous
MW (kDa):
205
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q86WI3
Entrez-Gene Id:
84166
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:100 |
Storage
Specificity/Sensitivity
Source / Purification
Background
Background References
- Kuenzel, S. et al. (2010) J Immunol 184, 1990-2000.
- Cui, J. et al. (2010) Cell 141, 483-96.
- Meissner, T.B. et al. (2010) Proc Natl Acad Sci USA 107, 13794-9.
- Meissner, T.B. et al. (2012) Microbes Infect 14, 477-84.
- Meissner, T.B. et al. (2012) J Immunol 188, 4951-8.
- Yoshihama, S. et al. (2016) Proc Natl Acad Sci USA 113, 5999-6004.
- Shukla, A. et al. (2021) Int J Mol Sci 22, 1964. doi: 10.3390/ijms22041964.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting W-S: Simple Western™ IP: Immunoprecipitation
Cross-Reactivity Key
H: Human M: Mouse R: Rat
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
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