Revision 1
#36303
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
15
Source/Isotype:
Rabbit IgG
UniProt ID:
#P42772
Entrez-Gene Id:
1030
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
p15 INK4B (E3R6S) Rabbit mAb recognizes endogenous levels of total p15 INK4B protein. This antibody does not cross-react with p16 INK4A.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg133 of human p15 INK4B protein.
Background
Members of the INK4 family of cyclin dependent kinase inhibitors include p16 INK4A, p15 INK4B, p18 INK4C, and p19 INK4D. The INK4 family members inhibit cyclin dependent kinases 4 and 6 (CDK4 and CDK6), causing cell cycle arrest in G1 phase. The INK4A-ARF-INK4B locus on chromosome 9p21, frequently lost in human cancer, encodes the INK4 family members p16 INK5A and p15 INK4B, as well as the unrelated protein, ARF (1).
p15 INK4B protein expression is induced by TGF-β in human keratinocytes (2), a process which requires demethylation at the p15 INK4B locus (3). In hematopoietic cells, p15 INK4B signaling functions in the determination of cell fate (4,5).
Researchers have shown that expression levels of p15 INK4B, altered by epigenetic modification, have an impact on disease progression in multiple myeloma (6). Similarly, studies in pancreatic cancer have shown hypermethylation and epigenetic silencing of p15 INK4B, p16 INK4A, and CDK inhibitors p21cip1 and p27kip1 (7).
Background References
- Kim, W.Y. and Sharpless, N.E. (2006) Cell 127, 265-75.
- Hannon, G.J. and Beach, D. (1994) Nature 371, 257-61.
- Thillainadesan, G. et al. (2012) Mol Cell 46, 636-49.
- Wolff, L. and Bies, J. (2013) Blood Cells Mol Dis 50, 227-31.
- Humeniuk, R. et al. (2013) Blood Cancer J 2, e99.
- Li, J. et al. (2014) Drug Des Devel Ther 8, 2129-36.
- Li, G. et al. (2012) Mol Med Rep 5, 1106-10.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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Limited Uses
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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
#36303
p15 INK4B (E3R6S) Rabbit mAb
Western blot analysis of extracts from HaCaT cells, untreated (-) or treated with Human Transforming Growth Factor β1 (hTGF-β1) #8915 (10 ng/mL, 18 hr; +), using p15 INK4B (E3R6S) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).