Phospho-Atg14 (Ser29) (D4B8M) Rabbit mAb #92340
- WB
- IF
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 65 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #41411.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
The serine/threonine kinase ULK1 phosphorylates Atg14 at Ser29 to promote autophagsome formation (13).
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- Zeng, X. et al. (2006) J Cell Sci 119, 259-70.
- Liang, C. et al. (2006) Nat Cell Biol 8, 688-99.
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- Sun, Q. et al. (2008) Proc Natl Acad Sci U S A 105, 19211-6.
- Zhong, Y. et al. (2009) Nat Cell Biol 11, 468-76.
- Matsunaga, K. et al. (2009) Nat Cell Biol 11, 385-96.
- Park, J.M. et al. (2016) Autophagy 12, 547-64.
Limited Uses
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