Revision 1
#14611
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IF-IC
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
230
Source/Isotype:
Rabbit IgG
UniProt ID:
#P35579
Entrez-Gene Id:
4627
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunofluorescence (Immunocytochemistry) | 1:50 |
Storage
Specificity/Sensitivity
Phospho-Myosin IIa (Ser1943) (D7Z7T) Rabbit mAb recognizes endogenous levels of myosin IIa protein only when phosphorylated at Ser1943.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1943 of human myosin II protein.
Background
Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Research studies have shown that phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7).
Background References
- Conti, M.A. and Adelstein, R.S. (2008) J Cell Sci 121, 11-18.
- Sandquist, J.C. et al. (2006) J Biol Chem 281, 35873-83.
- Even-Ram, S. et al. (2007) Nat Cell Biol 9, 299-309.
- Vicente-Manzanares, M. et al. (2007) J Cell Biol 176, 573-80.
- Wylie, S.R. and Chantler, P.D. (2008) Mol Biol Cell 19, 3956-68.
- Sandquist, J.C. and Means, A.R. (2008) Mol Biol Cell 19, 5156-67.
- Dulyaninova, N.G. et al. (2007) Mol Biol Cell 18, 3144-55.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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Revision 1
Western blot analysis of extracts from various cell lines, untreated (-) or treated with λ phosphatase and calf intestinal phosphatase (CIP; +), using Phospho-Myosin IIa (Ser1943) (D7Z7T) Rabbit mAb (upper) and Myosin IIa Antibody #3403 (lower).
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or λ phosphatase-treated (right), using Phospho-Myosin IIa (Ser1943) (D7Z7T) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).