Render Target: STATIC
Render Timestamp: 2024-12-20T12:12:33.810Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:10.066
Product last modified at: 2024-12-17T18:59:23.265Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

POM121 (E3W3J) Rabbit mAb #68775

Filter:
  • WB
  • IHC
  • IF

    Supporting Data

    REACTIVITY H M Mk
    SENSITIVITY Endogenous
    MW (kDa) 150
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:50 - 1:200
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    POM121 (E3W3J) Rabbit mAb recognizes endogenous levels of total POM121 protein. This antibody detects all POM121 protein variants, including POM121A, POM121B, and POM121C.

    Species Reactivity:

    Human, Mouse, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human POM121 protein.

    Background

    The nuclear pore complex (NPC) is a multi-subunit protein channel that spans the nuclear envelope and is responsible for the nucleocytoplasmic trafficking of RNA, proteins, and ribonucleoproteins (1,2). POM121, also referred to as nucleoporin Nup121, is a transmembrane nuclear protein that is an essential component of the NPC. POM121 expression is increased in aggressive, lethal prostate cancer and contributes to cancer progression and aggressiveness by enhancing the nuclear import of oncogenic transcription factors, such as E2F1, MYC, and GATA2, allowing for the upregulation of target genes (3). Elevated levels of POM121 have also been associated with poor outcome in patients with colorectal, lung, and laryngeal cancers, and it may serve as a prognostic biomarker for these cancer types (4-6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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