Revision 5
#79998
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, IHC-P
Reactivity:
H M R Mk
Sensitivity:
Endogenous
MW (kDa):
73
Source/Isotype:
Rabbit IgG
UniProt ID:
#O14744
Entrez-Gene Id:
10419
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Immunohistochemistry (Paraffin) | 1:400 - 1:1600 |
Storage
Specificity/Sensitivity
PRMT5 (D5P2T) Rabbit mAb recognizes endogenous levels of total PRMT5 protein. In Western blot analysis, the antibody also detects a 25 kDa protein of unknown identity.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human PRMT5 protein.
Background
Human Skb1Hs methyltransferase (also called JBP1), a homologue of yeast protein Skb1 and Hsl7p (1,2), is composed of 637 amino acid residues and contains motifs conserved among protein methyltransferases. It methylates histones and MBP in vitro (2). Yeast Hsl7p is involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p (3). An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast two-hybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK) (4). Both proteins belong to the protein methyltransferase superfamily (5). Interestingly, human Skb1Hs methyltransferase was shown to interact with Jak kinases. This suggests the possibility that the Skb1Hs methyltransferase could link Jak to a PAK signaling pathway in mammalian cells.
Background References
- Gilbreth, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 14781-14786.
- Pollack, B.P. et al. (1999) J. Biol. Chem. 274, 31531-31542.
- Ma, X.J. et al. (1996) Genes Dev. 10, 1327-1340.
- Gilbreth, M. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 13802-13807.
- Ma, X.J. et al. (2000) Trends Biochem. Sci. 25, 11-12.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
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