Revision 1
#25430
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
H M R Mk
Sensitivity:
Endogenous
MW (kDa):
97
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q13200
Entrez-Gene Id:
5708
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
PSMD2 (D6W7G) Rabbit mAb recognizes endogenous levels of total PSMD2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys350 of human PSMD2 protein.
Background
The 26S proteasome is a highly abundant proteolytic complex involved in the degradation of ubiquitinated substrate proteins. It consists largely of two sub-complexes, the 20S catalytic core particle (CP) and the 19S/PA700 regulatory particle (RP) that can cap either end of the CP. The CP consists of two stacked heteroheptameric β-rings (β1-7) that contain three catalytic β-subunits and are flanked on either side by two heteroheptameric α-rings (α1-7). The RP includes a base and a lid, each having multiple subunits. The base, in part, is composed of a heterohexameric ring of ATPase subunits belonging to the AAA (ATPases Associated with diverse cellular Activities) family. The ATPase subunits function to unfold the substrate and open the gate formed by the α-subunits, thus exposing the unfolded substrate to the catalytic β-subunits. The lid consists of ubiquitin receptors and DUBs that function in recruitment of ubiquitinated substrates and modification of ubiquitin chain topology (1,2). Other modulators of proteasome activity, such as PA28/11S REG, can also bind to the end of the 20S CP and activate it (1,2).
Regulatory particle non-ATPase 1 (RPN1, PSMD2) is a subunit of the 19S/PA700 regulatory particle base subcomplex. The PSMD2 protein acts as part of the scaffold for assembly of the 19S/PA700 RP base subcomplex (3). Research studies demonstrate that PSMD2 binds the intracellular domain of type I TNF receptor, indicating that the 26S proteasome may play a role in the TNF signaling pathway (4,5). PSMD2 expression correlates with poor prognosis in lung cancer patients, and induced inhibition of PSMD2 results in decreased proteasome activity and increased apoptosis in lung cancer cells (6).
Background References
- Finley, D. (2009) Annu Rev Biochem 78, 477-513.
- Lee, M.J. et al. (2011) Mol Cell Proteomics 10, R110.003871.
- Rosenzweig, R. et al. (2008) Nat Struct Mol Biol 15, 573-80.
- Tsurumi, C. et al. (1996) Eur J Biochem 239, 912-21.
- Dunbar, J.D. et al. (1997) J Immunol 158, 4252-9.
- Matsuyama, Y. et al. (2011) Mol Carcinog 50, 301-9.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
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Limited Uses
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Revision 1
#25430
PSMD2 (D6W7G) Rabbit mAb
Western blot analysis of extracts from various cell lines using PSMD2 (D6W7G) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human PSMD2 protein (hPSMD2-Myc/DDK; +), using PSMD2 (D6W7G) Rabbit mAb.