WB, IF-IC
H M R
Endogenous
18
Rabbit IgG
#P21246
5764
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:6400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PTN protein.
Background
Pleiotrophin (PTN) is a heparin-binding, developmentally regulated cytokine that is independently identified by several groups; it is also known as heparin-binding brain mitogen (HBBM), heparin-binding growth factor-8 (HBGF-8), heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), heparin-binding growth-associated molecule (HB-GAM), and osteoblast-specific factor 1 (OSF-1) (1-6). PTN is highly upregulated during embryonic development and early cell differentiation. In adulthood, PTN expression decreases in most tissues except for the bone and the nervous system. It interacts with numerous cell surface proteoglycan and non-proteoglycan receptors, including protein tyrosine phosphatase receptor type Z (PTPRZ), syndecans, nucleolin, neuropilin-1, ALK, and integrin αVβ3 and αMβ2 (reviewed in 7-9). PTN has been associated with biological events that involve cellular proliferation, differentiation, and inflammation, including tissue regeneration, bone repair, hypoxia, tumor growth, and angiogenesis.
- Kovesdi, I. et al. (1990) Biochem Biophys Res Commun 172, 850-4.
- Li, Y.S. et al. (1990) Science 250, 1690-4.
- Merenmies, J. and Rauvala, H. (1990) J Biol Chem 265, 16721-4.
- Tezuka, K. et al. (1990) Biochem Biophys Res Commun 173, 246-51.
- Rauvala, H. (1989) EMBO J 8, 2933-41.
- Vanderwinden, J.M. et al. (1992) Anat Embryol (Berl) 186, 387-406.
- Ballesteros-Pla, C. et al. (2023) Front Endocrinol (Lausanne) 14, 1225150.
- Wang, X. (2020) Adv Clin Chem 98, 51-89.
- Deuel, T.F. et al. (2002) Arch Biochem Biophys 397, 162-71.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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