Revision 6
#81254
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IHC-Bond, IHC-P
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
60-80
Source/Isotype:
Rabbit IgG
UniProt ID:
#P15151
Entrez-Gene Id:
5817
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
IHC Leica Bond | 1:100 - 1:400 |
Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #31235.
Specificity/Sensitivity
PVR/CD155 (D8A5G) Rabbit mAb recognizes endogenous levels of total CD155 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn188 of human CD155 protein.
Background
Poliovirus receptor (PVR), also known as CD155 and nectin-like 5 (Necl-5), is an immunoglobulin-like, transmembrane glycoprotein originally described as a mediator of poliovirus attachment to cells and later identified as important in adherens junction formation. PVR binds nectin-3 and interacts with integrin αvβ3 and PDGFR to regulate integrin clustering and focal contact formation at the leading edge of migrating cells (1,2). Research studies demonstrate that PVR and nectin-3 regulate contact inhibition during cell motility and proliferation in transformed 3T3 cells (3). Additional research indicates that PVR expression may play a role in the invasiveness of lung adenocarcinoma (4,5). In the immune system, CD155 plays a role in natural killer (NK) cell-mediated cytotoxicity (6).
Background References
- Takai, Y. et al. (2008) Nat Rev Mol Cell Biol 9, 603-15.
- Sakisaka, T. et al. (2007) Curr Opin Cell Biol 19, 593-602.
- Minami, Y. et al. (2007) Biochem Biophys Res Commun 352, 856-60.
- Tane, S. et al. (2013) Exp Mol Pathol 94, 330-5.
- Nakai, R. et al. (2010) Cancer Sci 101, 1326-30.
- Stanietsky, N. and Mandelboim, O. (2010) FEBS Lett 584, 4895-900.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IHC-Bond: IHC Leica Bond
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 6
#81254
PVR/CD155 (D8A5G) Rabbit mAb
Western blot analysis of extracts from various cell lines using PVR/CD155 (D8A5G) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using PVR/CD155 (D8A5G) Rabbit mAb performed on the Leica® Bond™ Rx.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PVR/CD155 (D8A5G) Rabbit mAb.
Revision 6
#81254
PVR/CD155 (D8A5G) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded A549 cell pellet (left, positive) and Daudi cell pellet (right, negative) using PVR/CD155 (D8A5G) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using PVR/CD155 (D8A5G) Rabbit mAb.