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#13075
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IF-IC
Reactivity:
H M R
Sensitivity:
Endogenous
MW (kDa):
22
Source/Isotype:
Rabbit IgG
UniProt ID:
#P62820
Entrez-Gene Id:
5861
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunofluorescence (Immunocytochemistry) | 1:800 |
Storage
Specificity/Sensitivity
Rab1A (D3X9S) Rabbit mAb recognizes endogenous levels of total Rab1A protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys191 of human Rab1A protein.
Background
Ras-related protein Rab1A (Rab1A) is a member of the Ras superfamily of cellular G proteins that function in protein transport and membrane restructuring (1). Early immunofluorescence studies determined that Rab1A localizes to a region between the endoplasmic reticulum (ER) and the Golgi complex, and in early Golgi compartments (2). Rab1A binds and recruits the COPII complex tethering factor p115 to a cis-SNARE complex associated with COPII-coated, budding vesicles on the endoplasmic reticulum (3). A Rab1 effector complex containing several proteins, including the cis-Golgi tethering protein GM130 and the stacking protein GRASP65, is essential for targeting and fusion of COPII-coated vesicles with the Golgi complex (4). Rab1A also interacts with the golgin tethering and docking proteins giantin (GOLGB1) and golgin-84 to regulate Golgi structure formation and function (5,6). Thus, Rab1A plays an important role in mediating the export of newly synthesized target proteins from ER to the Golgi. As with other Rab proteins, Rab1A function requires an intrinsic GTPase cycling activity facilitated by associated GEF and GAP factors (7-9). In addition to mediating ER to Golgi transport, Rab1A is also involved in autophagy during early autophagosome formation (10,11).
Background References
- Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
- Saraste, J. et al. (1995) J Cell Sci 108 ( Pt 4), 1541-52.
- Allan, B.B. et al. (2000) Science 289, 444-8.
- Moyer, B.D. et al. (2001) Traffic 2, 268-76.
- Koreishi, M. et al. (2013) PLoS One 8, e59821.
- Satoh, A. et al. (2003) Traffic 4, 153-61.
- Nuoffer, C. et al. (1994) J Cell Biol 125, 225-37.
- Pind, S.N. et al. (1994) J Cell Biol 125, 239-52.
- Haas, A.K. et al. (2007) J Cell Sci 120, 2997-3010.
- Huang, J. et al. (2011) Autophagy 7, 17-26.
- Lipatova, Z. et al. (2013) Mol Biol Cell , .
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
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