Revision 1
#13048
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, IF-IC
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
23
Source/Isotype:
Rabbit IgG
UniProt ID:
#P57735
Entrez-Gene Id:
57111
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Immunofluorescence (Immunocytochemistry) | 1:200 |
Storage
Specificity/Sensitivity
Rab25 (D4P6P) XP® Rabbit mAb recognizes endogenous levels of total Rab25 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rab25 protein.
Background
Rab11a, Rab11b, and Rab25 are members of the Rab11 subfamily of small Ras-like GTPases. Unlike universally expressed Rab11, typical Rab25 expression appears to be limited to gastrointestinal mucosa, kidney, and lung (1). Rab25 can associate with apical recycling vesicles to help regulate apical vesicle trafficking (2,3). Research studies indicate that atypical Rab25 expression can be associated with various forms of cancer. Increased Rab25 expression is associated with aggressive growth in ovarian and breast cancer, where Rab25 may inhibit apoptosis and promote cancer cell proliferation and invasion through regulation of vesicle transport and cellular motility (4-7). Interaction between Rab25 and β1 integrin promotes vesicle-mediated transport of integrin to pseudopodial tip membranes, fostering the persistent invasion of tumor cells (8). Conversely, the reported loss of Rab25 expression in a number of breast cancer cases has an unclear effect on cancer pathogenesis (9).
Background References
- Goldenring, J.R. et al. (1993) J Biol Chem 268, 18419-22.
- Casanova, J.E. et al. (1999) Mol Biol Cell 10, 47-61.
- Wang, X. et al. (2000) J Biol Chem 275, 29138-46.
- Cheng, K.W. et al. (2004) Nat Med 10, 1251-6.
- Cheng, K.W. et al. (2005) Cancer Res 65, 2516-9.
- Chia, W.J. and Tang, B.L. (2009) Biochim Biophys Acta 1795, 110-6.
- Tang, B.L. and Ng, E.L. (2009) Cell Motil Cytoskeleton 66, 365-70.
- Caswell, P.T. et al. (2007) Dev Cell 13, 496-510.
- Cheng, J.M. et al. (2010) Int J Cancer 126, 2799-812.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
#13048
Rab25 (D4P6P) XP® Rabbit mAb
Western blot analysis of extracts from various cell lines using Rab25 (D4P6P) XP® Rabbit mAb.
Western blot analysis of extracts from MCF7 (positive) and HeLa (negative) cells using Rab25 (D4P6P) XP® Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457 as a loading control.
Immunoprecipitation of Rab25 from ZR-75-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Rab25 (D4P6P) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Rab25 (D4P6P) XP® Rabbit mAb.
Revision 1
#13048
Rab25 (D4P6P) XP® Rabbit mAb
Confocal immunofluorescent analysis of MCF7 (positive, left) and HeLa (negative, right) cells using Rab25 (D4P6P) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).