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#18759
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
23
Source/Isotype:
Rabbit IgG
UniProt ID:
#O14966
Entrez-Gene Id:
8934
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity/Sensitivity
Rab29 (E4P6M) Rabbit mAb recognizes endogenous levels of total Rab29 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Rab29 protein.
Background
Rab29 (Rab7L1) is a member of the Ras GTPase superfamily of small monomeric G proteins responsible for regulation of intracellular membrane trafficking (1,2). Rab29 is involved in maintaining the integrity of the trans-Golgi network (TGN) and has been shown to play a role in the retrograde trafficking of mannose-6-phosphate receptors (M6PR) to the TGN (3). Rab29 is a substrate of LRRK2, a kinase associated with Parkinson’s disease (PD), and is one of five genes encoded within the PD-linked PARK16 locus (4,5). It has been shown that Rab29 recruits LRRK2 to the TGN and stimulates its kinase activity. Mimicking LRRK2 phosphorylation of Rab29 at Thr71 and Ser72 appears to suppress activation of LRRK2. Thus, Rab29 acts as a regulator of LRRK2, controlling its activation and localization (6).
Background References
- Li, G. and Marlin, M.C. (2015) Methods Mol Biol 1298, 1-15.
- Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
- Wang, S. et al. (2014) PLoS One 9, e96242.
- Steger, M. et al. (2016) Elife 5, e12813.
- Simón-Sánchez, J. et al. (2009) Nat Genet 41, 1308-12.
- Purlyte, E. et al. (2018) EMBO J 37, 1-18.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
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