Revision 3
#60386
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, eCLIP
Reactivity:
H M R Mk
Sensitivity:
Endogenous
MW (kDa):
110
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q96T37
Entrez-Gene Id:
64783
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
eCLIP | 1:200 |
Storage
Specificity/Sensitivity
RBM15 (E8Y8A) Rabbit mAb recognizes endogenous levels of total RBM15 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala557 of human RBM15 protein.
Background
RBM15 is an RNA-binding protein that is part of the WTAP-METTL3 m6A methyltransferase complex. RBM15 and related RBM15B interact with WTAP to recruit the complex to target mRNAs, and are critical to XIST-mediated gene silencing (1). RBM15 can recruit splicing factors such as SF3B1 to mRNA to promote alternative splicing. Expression levels of RBM15 can be regulated by PRMT1, which can methylate R578, resulting in RBM15 ubiquitinylation and degradation (2). This process is critical in acute megakaryoblastic leukemia, a cancer type where RBM15 is fused to the MKL-1 gene and PRMT1 is overexpressed (3). RBM15 normally plays roles in hematopoietic development and myeloid differentiation, where it can regulate the levels of c-Myc (4-6).
Background References
- Patil, D.P. et al. (2016) Nature 537, 369-73.
- Zhang, L. et al. (2015) Elife 4, e07938. doi: 10.7554/eLife.07938.
- Ma, Z. et al. (2001) Nat Genet 28, 220-1.
- Ma, X. et al. (2007) Mol Cell Biol 27, 3056-64.
- Raffel, G.D. et al. (2007) Proc Natl Acad Sci U S A 104, 6001-6.
- Niu, C. et al. (2009) Blood 114, 2087-96.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation eCLIP: eCLIP
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
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Limited Uses
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Revision 3
#60386
RBM15 (E8Y8A) Rabbit mAb
Western blot analysis of extracts from various cell lines using RBM15 (E8Y8A) Rabbit mAb.
Immunoprecipitation of RBM15 protein from 22Rv1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is RBM15 (E8Y8A) Rabbit mAb. Western blot analysis was performed using RBM15 (E8Y8A) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Enhanced cross-linking and immunoprecipitation (eCLIP) was performed with RNA from K-562 cells and RBM15 (E8Y8A) Rabbit mAb using a protocol based on the RBP-eCLIP method from Eclipsebio. The figure shows binding across the RBM15 transcript. Data is kindly provided by the laboratory of Dr. Gene Yeo and used with permission.