Revision 1
#99098
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
Vir
Sensitivity:
Endogenous
MW (kDa):
60
Source/Isotype:
Rabbit
Entrez-Gene Id:
43740578
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
Source / Purification
Background
SARS-CoV-2 3’-5’ exonuclease, or ExoN, is a non-structural protein (Nsp14) translated from ORF1b (2). It is a proofreading enzyme that functions in the replicase holoenzyme to read, excise, and repair errors in the newly transcribed viral RNA (3). RNA viruses generally have a high mutation rate and Coronaviridae, and others among the Nidovirales genus, have the largest and most complex genomes of RNA viruses (4). The 3’-5’ exonuclease is highly conserved between Coronaviruses, suggesting it’s important to the maintenance of such a large genome (5). The 3’-5’ exonuclease also provides a functionality to the RNA capping process, alongside the 2’-ribose methyl transferase (Nsp14), the helicase (Nsp13), and a guanylyl transferase (6,7). It is, however, the exonuclease function that is the key challenge to antiviral drug design with nucleoside analogue approaches (8).
Background References
- Zhou, P. et al. (2020) Nature 579, 270-273.
- Tortorici, M.A. and Veesler, D. (2019) Adv Virus Res 105, 93-116.
- Minskaia, E. et al. (2006) Proc Natl Acad Sci U S A 103, 5108-13.
- Saberi, A. et al. (2018) PLoS Pathog 14, e1007314.
- Denison, M.R. et al. RNA Biol 8, 270-9.
- Chen, Y. and Guo, D. (2016) Virol Sin 31, 3-11.
- Ma, Y. et al. (2015) Proc Natl Acad Sci U S A 112, 9436-41.
- Shannon, A. et al. (2020) Antiviral Res 178, 104793.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
Vir: Virus
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
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Revision 1