Revision 1
#57493
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
Vir
Sensitivity:
Endogenous
MW (kDa):
100
Source/Isotype:
Rabbit IgG
Entrez-Gene Id:
43740578
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
SARS-CoV-2 RNA-dependent RNA Polymerase (E4X1Z) Rabbit mAb recognizes endogenous levels of total SARS-CoV-2 RNA-dependent RNA polymerase protein in infected cells. In some lysates that were tested (non-Vero E6, non-infected), a faint, non-specific band around 110 kDa could be observed.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of SARS-CoV-2 RNA-dependent RNA polymerase protein.
Background
The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2).
SARS-CoV-2 RNA-dependent RNA polymerase, RdRp, is a non-structural protein (Nsp12) translated from ORF1b. It is the key enzyme essential for transcription of viral RNA from RNA templates and is therefore critical to replication of the virus (2). The polymerase has a structure similar to all viral RNA RdRps, the so-called hand structure: the catalytic module consists of palm, fingers, and thumb domains, forming the encircled human right hand model. Some conservation exists between different RNA viruses in the palm and finger domains, but there are significant differences in other domains reflecting different catalytic modes of action (3). SARS-CoV-2 RdRp requires accessory proteins, Nsp7 and Nsp8, along with Nsp14 for transcriptional activity (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
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