Revision 1
#33443
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, IHC-P
Reactivity:
H M R Mk
Sensitivity:
Endogenous
MW (kDa):
52
Source/Isotype:
Rabbit IgG
UniProt ID:
#P34897
Entrez-Gene Id:
6472
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
Specificity/Sensitivity
SHMT2 (E7F4Q) Rabbit mAb recognizes endogenous levels of total SHMT2 protein. This antibody does not cross-react with SHMT1 protein. Species cross-reactivity for IHC-P is human only.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly451 of human SHMT2 protein.
Background
Serine hydroxymethyltransferases 1 and 2 (SHMT1, SHMT2) are cytoplasmic and mitochondrial serine hydroxymethyltransferases, respectively (1,2). They catalyze the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate (THF) to form 5, 10-methylene-THF (1, 2). Research studies indicate that SHMT1 hemizygosity is associated with higher risk of intestinal cancer in mice of a certain genetic background (3). Suppression of SHMT2 was shown to block cell proliferation (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
#33443
SHMT2 (E7F4Q) Rabbit mAb
Western blot analysis of extracts from RH-30, MDA-MB-231, and HeLa cells using SHMT2 (E7F4Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of SHMT2 protein from RH-30 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SHMT2 (E7F4Q) Rabbit mAb. Western blot analysis was performed using SHMT2 (E7F4Q) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SHMT2 (E7F4Q) Rabbit mAb.
Revision 1
#33443
SHMT2 (E7F4Q) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using SHMT2 (E7F4Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human mucoepidermoid carcinoma of the larynx using SHMT2 (E7F4Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using SHMT2 (E7F4Q) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Revision 1
#33443
SHMT2 (E7F4Q) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using SHMT2 (E7F4Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human granulosa cell tumor of the ovary using SHMT2 (E7F4Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using SHMT2 (E7F4Q) Rabbit mAb (left) or SHMT2 (E8O8J) Rabbit mAb (IHC Specific) #37004 (right). These two antibodies detect independent, unique epitopes on human SHMT2. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Revision 1
#33443
SHMT2 (E7F4Q) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded RH-30 cell pellet (left, high-expressing) or MDA-MB-231 cell pellet (right, low-expressing) using SHMT2 (E7F4Q) Rabbit mAb.