WB, IP
H
Endogenous
80-100
Rabbit IgG
#Q9Y286
27036
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met395 of human Siglec-7 protein.
Background
Siglec-7 (Sialic acid-binding Ig-like lectin 7, AIRM1) is a member of the CD33-related Siglec family, a subset of the immunoglobulin superfamily of cell surface proteins (1-3). Siglec-7 is found in human immune cells such as natural killer (NK) cells, monocytes, and neutrophils (1,2). Siglec-7 acts as an immune inhibitory receptor via its intracellular Tyrosine-based Inhibitory Motif (ITIM) (1-5). Downregulation of cell surface Siglec-7 is an early marker of NK cell dysfunction, which can be associated with chronic viral infection or impaired anti-tumor activity (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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