Revision 1
#68631
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
23
Source/Isotype:
Rabbit IgG
UniProt ID:
#O15524
Entrez-Gene Id:
8651
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Storage
Specificity/Sensitivity
SOCS1 (E4K7Q) Rabbit mAb recognizes endogenous levels of total SOCS1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SOCS1 protein.
Background
The suppressor of cytokine signaling (SOCS) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway (1-3). The SOCS family consists of at least 8 members including the originally identified cytokine-inducible SH2-containing protein (CIS1), as well as SOCS1-7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses.
SOCS1 (suppressor of cytokine signaling 1), also known as JAB (Janus Kinase binding protein), SSI-1 (Stat-induced Stat inhibitor-1), and TIP3 (Tec-interacting protein 3) (4-7), is a cytokine-regulated SOCS family member that directly inhibits Jak family members through interaction within their kinase activation loop. In addition to inhibiting Jak/Stat signaling, SOCS1 can also negatively regulate Toll-like receptors that contribute to innate immunity (8,9). The SOCS box of SOCS1 can trigger ubiquitin-mediated degradation of proteins within and outside of the Jak/Stat pathway (10,11). The highest expression of SOCS1 is seen in the thymus and spleen and it plays a critical role in T cell activation and lymphocyte differentiation (4,12). SOCS1 also functions as a tumor suppressor protein by inhibiting hematopoietic oncogenes (13,14).
Background References
- Alexander, W.S. et al. (1999) J Leukoc Biol 66, 588-92.
- Chen, X.P. et al. (2000) Immunity 13, 287-90.
- Hilton, D.J. et al. (1998) Proc Natl Acad Sci USA 95, 114-9.
- Starr, R. et al. (1997) Nature 387, 917-21.
- Endo, T.A. et al. (1997) Nature 387, 921-4.
- Naka, T. et al. (1997) Nature 387, 924-9.
- Ohya, K.i. et al. (1997) J Biol Chem 272, 27178-82.
- Kinjyo, I. et al. (2002) Immunity 17, 583-91.
- Davey, G.M. et al. (2006) Tissue Antigens 67, 1-9.
- Kamizono, S. et al. (2001) J Biol Chem 276, 12530-8.
- Rui, L. et al. (2002) J Biol Chem 277, 42394-8.
- Marine, J.C. et al. (1999) Cell 98, 609-16.
- De Sepulveda, P. et al. (1999) EMBO J 18, 904-15.
- Rottapel, R. et al. (2002) Oncogene 21, 4351-62.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
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Revision 1
#68631
SOCS1 (E4K7Q) Rabbit mAb
Western blot analysis of extracts from various cell lines using SOCS1 (E4K7Q) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The absence of signal for SOCS1 in U266B1 cells has been found to be caused by aberrant hypermethylation of the SOCS1 gene and confirms the specificity of the antibody for SOCS1.
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human SOCS1 protein (hSOCS1-Myc/DDK; +), using SOCS1 (E4K7Q) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Immunoprecipitation of SOCS1 protein from L-540 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 SOCS1 (E4K7Q) Rabbit mAb. Western blot analysis was performed using SOCS1 (E4K7Q) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.