Revision 1
#93130
Store at -20C
Stat Antibody Sampler Kit II
1 Kit
(6 x 20 microliters)
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Stat1 (D1K9Y) Rabbit mAb | 14994 | 20 µl | 84, 91 kDa | Rabbit IgG |
| Stat2 (D9J7L) Rabbit mAb | 72604 | 20 µl | 97, 113 kDa | Rabbit IgG |
| Stat3 (D1B2J) Rabbit mAb | 30835 | 20 µl | 79, 86 kDa | Rabbit IgG |
| Stat4 (C46B10) Rabbit mAb | 2653 | 20 µl | 81 kDa | Rabbit |
| Stat5 (D2O6Y) Rabbit mAb | 94205 | 20 µl | 90 kDa | Rabbit IgG |
| Stat6 (D3H4) Rabbit mAb | 5397 | 20 µl | 110 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
Stat Antibody Sampler Kit II provides an economical means to examine the complete Stat family: Stat1-6. The kit contains enough a primary antibody to perform two western blot experiments with each primary antibody.
Storage
Background
Janus kinases (Jaks) and signal transducers and activators of transcription (Stats) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors, and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules, and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to interferon response element (IRE) and gamma interferon-activated sequence (GAS) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats (2,3), and by the combinatorial coupling of various Stat members to different receptors. Serine phosphorylation in the carboxy-terminal transcriptional activation domain has been shown to regulate the function of Stat1, Stat2, Stat3, Stat4, and Stat5 (1). Phosphorylation of Stat3 at Ser727 via MAPK or mTOR pathways is required for optimal transcriptional activation in response to growth factors and cytokines including IFN-gamma and ciliary neurotrophic factor (CNTF) (4,5). Jak/Stat pathways also play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses, and stem cell differentiation (6-11).
Background References
- Darnell Jr., J. et al. (1994) Science 264, 1415-1421.
- Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
- Caldenhoven, E. et al. (1996) J. Biol. Chem. 271, 13221-13227.
- Wen, Z. et al. (1995) Cell 82, 241-250.
- Yokogami, K. et al. (2000) Curr. Biol. 10, 47-50.
- Lim, C.P. and Cao, X. (1999) J. Biol. Chem. 274, 31055-31061.
- Bromberg, J. F. et al. (1999) Cell 98, 295-303.
- Su, L. et al. (1999) J. Biol. Chem. 274, 31770-31774.
- Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.
- Cattaneo, E. et al. (1999) Trends Neurosci. 22, 365-369.
- Frank, D.A. (1999) Mol. Med. 5, 432-456.
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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Limited Uses
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