Revision 1
#71192
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP
Reactivity:
H M R Mk
Sensitivity:
Endogenous
MW (kDa):
55
Source/Isotype:
Mouse IgG2a
UniProt ID:
#Q9GZV5
Entrez-Gene Id:
25937
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity/Sensitivity
TAZ (E5P2N) Mouse mAb recognizes endogenous levels of total TAZ protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro49 of human TAZ protein.
Background
TAZ is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 proteins (1). TAZ shares homology with the WW domain of Yes-associated protein (YAP) (1). TAZ is proposed to modulate the switch between proliferation and differentiation of mesenchymal stem cells (MSC) via interaction with transcription factors Runx2 and PPARγ. This process is critical to normal tissue development and the prevention of tumor formation. Due to its role in determination of MSC fate, TAZ may have clinical relevance to several human diseases caused by an imbalance of MSC differentiation (2,3). TAZ is negatively regulated via phosphorylation by LATS1/2, core kinases in the Hippo signaling pathway that controls stem cell development, tissue growth and tumor development (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
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Limited Uses
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Revision 1
#71192
TAZ (E5P2N) Mouse mAb
Western blot analysis of extracts from various cell lines using TAZ (E5P2N) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). TAZ expression is undetectable in RL extracts, as predicted from published gene expression databases.
Immunoprecipitation of TAZ from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Mouse (E5Y6Q) mAb IgG2a Isotype Control #61656, and lane 3 is TAZ (E5P2N) Mouse mAb. Western blot analysis was performed using TAZ (D3I6D) Rabbit mAb #70148. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody.