ChIP
H
Endogenous
Rabbit IgG
#P15884
6925
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Chromatin IP | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Bovine, Dog, Pig, Horse, Rabbit
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro653 of human TCF4/ITF2 protein.
Background
Transcription factor 4 (TCF4), also known as immunoglobulin transcription factor 2 (ITF2), is a member of the basic helix-loop-helix family and interacts with Ephrussi-box (E-box) binding sites (1,2). TCF4 is broadly expressed, and due to alternative splicing, transcripts can give rise to over a dozen different isoforms (3). It plays a key role in neuronal development and has been implicated in a variety of developmental disorders, including Pitt-Hopkins syndrome, schizophrenia, and autism (4-9). In addition to its functions in the brain, TCF4 is essential for the development of plasmacytoid dendritic cells (10-12). Finally, in the context of cancer, TCF4 expression may be associated with divergent patient outcomes; higher TCF4 expression is associated with increased survival in non-small cell lung cancer, yet the protein has also recently been reported to confer immunotherapy resistance in melanoma (13,14).
- Murre, C. et al. (1994) Biochim Biophys Acta 1218, 129-35.
- Furumura, M. et al. (2001) J Biol Chem 276, 28147-54.
- Sepp, M. et al. (2011) PLoS One 6, e22138.
- Flora, A. et al. (2007) Proc Natl Acad Sci USA 104, 15382-7.
- Brockschmidt, A. et al. (2007) Hum Mol Genet 16, 1488-94.
- Rannals, M.D. and Maher, B.J. (2017) Curr Genet Med Rep 5, 1-7.
- Stefansson, H. et al. (2009) Nature 460, 744-7.
- Mesman, S. et al. (2020) Mol Cell Neurosci 106, 103502.
- Papes, F. et al. (2022) Nat Commun 13, 2387.
- Nagasawa, M. et al. (2008) Eur J Immunol 38, 2389-400.
- Cisse, B. et al. (2008) Cell 135, 37-48.
- Dewald, H.K. et al. (2020) Viruses 12, 162.
- Pernía, O. et al. (2020) Cancers (Basel) 12, 786.
- Pozniak, J. et al. (2024) Cell 187, 166-183.e25.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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