Revision 4
#99980
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP
Reactivity:
H M R Mk
Sensitivity:
Endogenous
MW (kDa):
235
Source/Isotype:
Rabbit IgG
UniProt ID:
#O43151
Entrez-Gene Id:
200424
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Storage
Specificity/Sensitivity
TET3 (E6J8A) Rabbit mAb recognizes endogenous levels of total TET3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human TET3 protein.
Background
Methylation of DNA at cytosine residues is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and mammalian development (1,2). 5-methylcytosine is a repressive epigenetic mark established de novo by two enzymes, DNMT3a and DNMT3b, and is maintained by DNMT1 (3,4). 5-methylcytosine was originally thought to be passively depleted during DNA replication. However, subsequent studies have shown that Ten-Eleven Translocation (TET) proteins TET1, TET2, and TET3 can catalyze the oxidation of methylated cytosine to 5-hydroxymethylcytosine (5-hmC) (5). Additionally, TET proteins can further oxidize 5-hmC to form 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC), both of which are excised by thymine-DNA glycosylase (TDG), effectively linking cytosine oxidation to the base excision repair pathway and supporting active cytosine demethylation (6,7). TET3 plays several key roles in regulating early development and neonatal growth. First, TET3 functions to demethylate DNA in the male pronucleus of the zygote following fertilization (8-10). In addition, TET3 binds to and regulates numerous developmental genes later during development (11). TET2/TET3 deficiency can lead to myeloid cell, B cell, and invariant natural killer T (iNKT) cell malignancies. In Tregs, TET2/TET3 deficiency in mice leads to hyperproliferation and inflammatory disease, with decreased expression of Treg-specific genes and increased expression of genes involved in proliferation and cancer (12,13).
Background References
- Hermann, A. et al. (2004) Cell Mol Life Sci 61, 2571-87.
- Turek-Plewa, J. and Jagodziński, P.P. (2005) Cell Mol Biol Lett 10, 631-47.
- Okano, M. et al. (1999) Cell 99, 247-57.
- Li, E. et al. (1992) Cell 69, 915-26.
- Tahiliani, M. et al. (2009) Science 324, 930-5.
- He, Y.F. et al. (2011) Science 333, 1303-7.
- Ito, S. et al. (2011) Science 333, 1300-3.
- Peat, J.R. et al. (2014) Cell Rep 9, 1990-2000.
- Inoue, A. et al. (2015) Cell Rep 10, 463-70.
- Tsukada, Y. et al. (2015) Sci Rep 5, 15876.
- Xu, Y. et al. (2012) Cell 151, 1200-13.
- Nakatsukasa, H. et al. (2019) Int Immunol 31, 335-47.
- Yue, X. et al. (2019) Nat Commun 10, 2011.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
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Revision 4
#99980
TET3 (E6J8A) Rabbit mAb
Western blot analysis of extracts from various cell lines using TET3 (E6J8A) Rabbit mAb.
Immunoprecipitation of TET3 protein from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TET3 (E6J8A) Rabbit mAb. Western blot analysis was performed using TET3 (E6J8A) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody. Immunoprecipitation with primary antibody was performed for two hours at 4°C.