Revision 4
#99543
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, IF-IC
Reactivity:
H M R Mk
Sensitivity:
Endogenous
MW (kDa):
70
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q9H1C4
Entrez-Gene Id:
81622
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:200 |
Storage
Specificity/Sensitivity
Source / Purification
Background
Background References
- Tabeta, K. et al. (2006) Nat Immunol 7, 156-64.
- Kim, Y.M. et al. (2008) Nature 452, 234-8.
- Pelka, K. et al. (2014) J Immunol 193, 3257-61.
- Lee, B.L. et al. (2013) Elife 2, e00291.
- Fukui, R. et al. (2011) Immunity 35, 69-81.
- Majer, O. et al. (2019) Nature 575, 366-370.
- Pelka, K. et al. (2018) Immunity 48, 911-922.e7.
- Majer, O. et al. (2019) Nature 575, 371-374.
- Zhu, H. et al. (2022) J Med Virol 94, 4490-4501.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Mk: Monkey
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
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