Revision 1
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
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Product Information

Product Usage Information

Use with a Peptide Desalting C18 StageTip for processing peptide samples prior to liquid chromatography-mass spectrometry (LCMS). Dimensions of microcentrifuge tubes and centrifuge devices can vary, and we recommend a microcentrifuge tube that can accommodate the inner diameter of the adaptor and a centrifuge that can accommodate the tube, adaptor, and StageTip with a rotor lid. Below are examples of equipment we have tested that fit the provided adaptor.

Recommended equipment (not provided):
Posi-Click 1.5 mL graduated microcentrifuge tube (Thomas Scientific)
Peptide Desalting C18 StageTip (Cell Signaling Technology, #45943)
MiniSpin Centrifuge with F-45-12-11 rotor (Eppendorf)

1) Remove the lid from a clean 1.5 mL microcentrifuge tube.

2) Insert an adaptor inside the microcentrifuge tube.

3) Insert a Peptide Desalting C18 StageTip through the central hole in the adaptor. The StageTip should sit easily inside the adaptor. There should be enough space between the bottom of the microcentrifuge tube and the bottom of the StageTip to accommodate at least 100 μL of liquid. Users can proceed with peptide desalting.

4) After experiments are finished, users can wipe clean the adaptor with 70% ethanol and air dry it if necessary. DO NOT use detergent- or soap-containing solutions to clean the adaptor. Users can store the adaptor for re-use at room temperature in a closed bag or box to minimize dust exposure.

Storage

Store at room temperature.

Product Description

The Peptide Desalting C18 StageTip Adaptor is a reusable option to vertically position a C18 StageTip inside a microcentrifuge tube, allowing for removal of wash and waste solutions and for collection of desalted peptide elutions.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.