Revision 1
#39172
Store at -20C
CD11b/ITGAM (E4K8C) & CO-0083-488 SignalStar™ Oligo-Antibody Pair
1 Kit
(10 slides)
UniProt ID:
#P05555
Entrez-Gene Id:
#16409
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
| Product Includes | Product # | Volume | Reactivity | Isotype |
|---|---|---|---|---|
| CD11b/ITGAM (E4K8C) Rabbit mAb (SignalStar™ Conjugate 0083) | 86325 | 50 µl | M | Rabbit IgG |
| Complementary Oligo (CO-0083-488) | 58938 | 22 µl |
Storage
Description
SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
Specificity/Sensitivity
CD11b/ITGAM (E4K8C) Rabbit mAb (SignalStar™ Conjugate 0083) recognizes endogenous levels of total CD11b/ITGAM protein. Non-specific staining was observed in mouse pancreatic acinar cells by immunohistochemistry.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser832 of mouse CD11b/ITGAM protein.
Background
Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (1). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (2). CD11b is phosphorylated at Ser1126 (cytoplasmic tail) in neutrophils. Research has shown that this phosphorylation event plays a role for leukocytes traveling from the bloodstream to tissues (3). Furthermore, genome-wide association studies have linked CD11b to autoimmune diseases, such as systemic lupus erythematosus (SLE) (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
StarBond: SignalStar™ Leica Bond
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStar is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 10,781,477, foreign equivalents, and child patents deriving therefrom.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded mouse lung using CD11b/ITGAM (E4K8C) & CO-0083-594 SignalStar™ Oligo-Antibody Pair #84898 (yellow), CD39/NTPDase 1 (E2X6B) & CO-0076-647 SignalStar™ Oligo-Antibody Pair #88633 (magenta), Pan-Keratin (Type I) (E6S1S) & CO-0072-750 SignalStar™ Oligo-Antibody Pair #53360 (cyan), CD3ε (E4T1B) & CO-0048-488 SignalStar™ Oligo-Antibody Pair #92858 (green), F4/80 (D2S9R) & CO-0042-750 SignalStar™ Oligo-Antibody Pair #51924 (red), and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using CD11b/ITGAM (E4K8C) & CO-0083-488 SignalStar™ Oligo-Antibody Pair #39172 (green). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded mouse lung using CD11b/ITGAM (E4K8C) & CO-0083-594 SignalStar™ Oligo-Antibody Pair #84898 (yellow). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Revision 1
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded mouse thymus using CD11b/ITGAM (E4K8C) & CO-0083-647 SignalStar™ Oligo-Antibody Pair #27353 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded LL/2 syngeneic tumor using CD11b/ITGAM (E4K8C) & CO-0083-750 SignalStar™ Oligo-Antibody Pair #56034 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded LL/2 syngeneic tumor using CD11b/ITGAM (E4K8C) & CO-0083-750 SignalStar™ Oligo-Antibody Pair #56034 (left, cyan) and DAPI #4083 (left, blue) compared to chromogenic immunohistochemical analysis of a serial section of paraffin-embedded LL/2 syngeneic tumor using CD11b/ITGAM (E4K8C) Rabbit mAb #93169 (right). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Revision 1
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded GL261 syngeneic tumor using CD11b/ITGAM (E4K8C) & CO-0083-488 SignalStar™ Oligo-Antibody Pair #39172 (green), CD36 (E8B7S) & CO-0089-594 SignalStar™ Oligo-Antibody Pair #23418 (yellow), Pan-Keratin (Type I) (E6S1S) & CO-0072-647 SignalStar™ Oligo-Antibody Pair #77030 (red), IL-2Rα/CD25 (E9W2J) & CO-0074-750 SignalStar™ Oligo-Antibody Pair #71915 (magenta), CD8α (D4W2Z) & CO-0040-488 SignalStar™ Oligo-Antibody Pair #17707 (white), Arginase-1 (D4E3M) & CO-0075-594 SignalStar™ Oligo-Antibody Pair #66757 (cyan), F4/80 (D2S9R) & CO-0042-750 SignalStar™ Oligo-Antibody Pair #51924 (orange), and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mammary tumor using CD36 (E8B7S) & CO-0089-488 SignalStar™ Oligo-Antibody Pair #95886 (green), CD11b/ITGAM (E4K8C) & CO-0083-594 SignalStar™ Oligo-Antibody Pair #84898 (yellow), CD39/NTPDase 1 (E2X6B) & CO-0076-647 SignalStar™ Oligo-Antibody Pair #88633 (red), Pan-Keratin (Type I) (E6S1S) & CO-0072-750 SignalStar™ Oligo-Antibody Pair #53360 (cyan), and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated.
