Revision 1
#56104
Store at -20C
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) & CO-0107-750 SignalStar™ Oligo-Antibody Pair
1 Kit
(10 slides)
UniProt ID:
#P62753
Entrez-Gene Id:
#6194
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
| Product Includes | Product # | Volume | Reactivity | Isotype |
|---|---|---|---|---|
| Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (SignalStar™ Conjugate 0107) | 37573 | 50 µl | H M R Mk Mi Sc | Rabbit IgG |
| Complementary Oligo (CO-0107-750) | 17124 | 22 µl |
Storage
Description
SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
Specificity/Sensitivity
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (SignalStar™ Conjugate 0107) detects endogenous levels of S6 ribosomal protein only when phosphorylated at Ser235 and 236. Non-specific labeling in pancreatic islets has been observed by immunofluorescence in fixed frozen mouse tissue post-processed with λ phosphatase.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human S6 ribosomal protein.
Background
One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5).
Background References
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
StarBond: SignalStar™ Leica Bond
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStar is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 10,781,477, foreign equivalents, and child patents deriving therefrom.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
#56104
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) & CO-0107-750 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded 4T1 syngeneic tumor using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) & CO-0107-488 SignalStar™ Oligo-Antibody Pair #71437 (green). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) & CO-0107-594 SignalStar™ Oligo-Antibody Pair #92253 (yellow). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human colorectal adenocarcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) & CO-0107-647 SignalStar™ Oligo-Antibody Pair #18602 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Revision 1
#56104
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) & CO-0107-750 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) & CO-0107-750 SignalStar™ Oligo-Antibody Pair #56104 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) & CO-0107-594 SignalStar™ Oligo-Antibody Pair #92253 (left, yellow) and DAPI #4083 (left, blue) compared to chromogenic immunohistochemical analysis of a serial section of paraffin-embedded human squamous cell lung carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (right). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using COL1A1 (E8F4L) & CO-0106-488 SignalStar™ Oligo-Antibody Pair #56221 (green), Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) & CO-0107-594 SignalStar™ Oligo-Antibody Pair #92253 (yellow), FAP (F1A4G) & CO-0116-488 SignalStar™ Oligo-Antibody Pair #76731 (lavender), β-Catenin (D10A8) & CO-0108-594 SignalStar™ Oligo-Antibody Pair #83149 (orange), CD40 (D8W3N) & CO-0115-647 SignalStar™ Oligo-Antibody Pair #39704 (red), Pan-Keratin (C11) & CO-0003-750 SignalStar™ Oligo-Antibody Pair #97227 (magenta), and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Revision 1
#56104
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) & CO-0107-750 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human tonsil using CD40 (D8W3N) & CO-0115-594 SignalStar™ Oligo-Antibody Pair #23671 (yellow), COL1A1 (E8F4L) & CO-0106-647 SignalStar™ Oligo-Antibody Pair #87785 (red), Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) & CO-0107-750 SignalStar™ Oligo-Antibody Pair #56104 (cyan), and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human colorectal adenocarcinoma using FAP (F1A4G) & CO-0116-594 SignalStar™ Oligo-Antibody Pair #91349 (yellow), Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) & CO-0107-647 SignalStar™ Oligo-Antibody Pair #18602 (red), and COL1A1 (E8F4L) & CO-0106-750 SignalStar™ Oligo-Antibody Pair #55257 (cyan). All fluorophores have been assigned a pseudocolor, as indicated.
