Revision 1

#23418Store at -20C

1 Kit

(10 slides)

UniProt ID:

#Q08857

Entrez-Gene Id:

#12491

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Item # Volume Reactivity Isotype
CD36 (E8B7S) Rabbit mAb (SignalStar Conjugate 0089) 28617 50 µl M Rabbit IgG
Complementary Oligo (CO-0089-594) 33183 22 µl

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Specificity/Sensitivity

CD36 (E8B7S) Rabbit mAb (SignalStar Conjugate 0089) recognizes endogenous levels of total CD36 protein and reacts with an epitope surrounding Ile410. Non-specific nucleolar background may be observed by immunofluorescence in methanol fixed samples.

Source / Purification

Monoclonal antibody is produced by immunizing animals with mouse CD36 recombinant protein.

Background

CD36 is a class B scavenger receptor composed of short amino-terminal and carboxy-terminal cytoplasmic domains, two transmembrane domains, and a large glycosylated extracellular domain (1-4). The CD36 receptor has many diverse ligands and cellular functions and is expressed by multiple cell types, including monocytes, macrophages, platelets, endothelial cells, adipocytes, and some epithelial cells (1). Binding of thrombospondin-1 (TSP-1) to CD36 facilitates the inhibition of angiogenesis by TSP-1 (5). CD36 also binds lipids and enables their transport into cells (6). In macrophages, CD36 acts as a receptor for oxidized LDL (Ox-LDL) and is responsible for Ox-LDL internalization, which contributes to development of atherosclerosis (7). The CD36 receptor participates in the innate immune response by acting as a pattern recognition receptor for lipid components of bacterial cell walls and fungal beta-glucans (8,9). CD36 likely influences signaling by interacting with other cell surface receptors including TLRs, integrins, and tetraspanins (8,10,11). Phorbol 12-myristate 13-acetate (PMA)/ 12-O-tetradecanoylphorbol-13-acetate (TPA) induces CD36 expression in the THP-1 monocyte cell line (12).

  1. Asch, A.S. et al. (1987) J Clin Invest 79, 1054-61.
  2. Armesilla, A.L. and Vega, M.A. (1994) J Biol Chem 269, 18985-91.
  3. Tandon, N.N. et al. (1989) J Biol Chem 264, 7570-5.
  4. Endemann, G. et al. (1993) J Biol Chem 268, 11811-6.
  5. Dawson, D.W. et al. (1997) J Cell Biol 138, 707-17.
  6. Kim, Y.R. and Peterkofsky, B. (1997) J Cell Biochem 67, 338-52.
  7. Nicholson, A.C. et al. (1995) Arterioscler Thromb Vasc Biol 15, 269-75.
  8. Hoebe, K. et al. (2005) Nature 433, 523-7.
  9. Means, T.K. et al. (2009) J Exp Med 206, 637-53.
  10. Mattera, M.D. (1992) RN 55, 7.
  11. Miao, W.M. et al. (2001) Blood 97, 1689-96.
  12. Alessio, M. et al. (1996) J Biol Chem 271, 1770-5.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStar is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 10,781,477, foreign equivalents, and child patents deriving therefrom.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Revision 1
#23418

CD36 (E8B7S) & CO-0089-594 SignalStar Oligo-Antibody Pair

Immunohistochemistry Image 1: CD36 (E8B7S) & CO-0089-594 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar multiplex immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mammary tumor using CD36 (E8B7S) & CO-0089-488 SignalStar Oligo-Antibody Pair #95886 (green), CD11b/ITGAM (E4K8C) & CO-0083-594 SignalStar Oligo-Antibody Pair #84898 (magenta), Pan-Keratin (Type I) (E6S1S) & CO-0072-750 SignalStar Oligo-Antibody Pair #53360 (cyan), and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 2: CD36 (E8B7S) & CO-0089-594 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar multiplex immunohistochemical analysis of paraffin-embedded mouse lung using CD36 (E8B7S) & CO-0089-488 SignalStar Oligo-Antibody Pair #95886 (green). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 3: CD36 (E8B7S) & CO-0089-594 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar multiplex immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using CD36 (E8B7S) & CO-0089-594 SignalStar Oligo-Antibody Pair #23418 (yellow). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 4: CD36 (E8B7S) & CO-0089-594 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar multiplex immunohistochemical analysis of paraffin-embedded mouse spleen using CD36 (E8B7S) & CO-0089-647 SignalStar Oligo-Antibody Pair #52907 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 5: CD36 (E8B7S) & CO-0089-594 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar multiplex immunohistochemical analysis of paraffin-embedded mouse thymus using CD36 (E8B7S) & CO-0089-750 SignalStar Oligo-Antibody Pair #70491 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 6: CD36 (E8B7S) & CO-0089-594 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar multiplex immunohistochemical analysis of paraffin-embedded mouse lung using CD36 (E8B7S) & CO-0089-488 SignalStar Oligo-Antibody Pair #95886 (left, green) and DAPI #4083 (left, blue) compared to chromogenic immunohistochemical analysis of a serial section of paraffin-embedded mouse lung using CD36 (E8B7S) Rabbit mAb  #28109 (right). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 7: CD36 (E8B7S) & CO-0089-594 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar multiplex immunohistochemical analysis of paraffin-embedded GL261 syngeneic tumor using CD11b/ITGAM (E4K8C) & CO-0083-488 SignalStar Oligo-Antibody Pair #39172 (green), CD36 (E8B7S) & CO-0089-594 SignalStar Oligo-Antibody Pair #23418 (yellow), Pan-Keratin (Type I) (E6S1S) & CO-0072-647 SignalStar Oligo-Antibody Pair #77030 (red), IL-2Rα/CD25 (E9W2J) & CO-0074-750 SignalStar Oligo-Antibody Pair #71915 (magenta), CD8α (D4W2Z) & CO-0040-488 SignalStar Oligo-Antibody Pair #17707 (white), Arginase-1 (D4E3M) & CO-0075-594 SignalStar Oligo-Antibody Pair #66757 (cyan), F4/80 (D2S9R) & CO-0042-750 SignalStar Oligo-Antibody Pair #51924 (orange), and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 1: CD36 (E8B7S) & CO-0089-594 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar multiplex immunohistochemical analysis of paraffin-embedded mouse spleen using CD39/NTPDase 1 (E2X6B) & CO-0076-594 SignalStar Oligo-Antibody Pair #71665 (red), CD36 (E8B7S) & CO-0089-647 SignalStar Oligo-Antibody Pair #52907 (yellow), CD11b/ITGAM (E4K8C) & CO-0083-750 SignalStar Oligo-Antibody Pair #56034 (magenta), and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated.