Revision 1
#37369
Store at -20C
Granzyme B (D6E9W) & CO-0009-647 SignalStar™ Oligo-Antibody Pair
1 Kit
(10 slides)
UniProt ID:
#P10144
Entrez-Gene Id:
#3002
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
| Product Includes | Product # | Volume | Reactivity | Isotype |
|---|---|---|---|---|
| Granzyme B (D6E9W) Rabbit mAb (SignalStar™ Conjugate 0009) | 74167 | 50 µl | H | Rabbit IgG |
| Complementary Oligo (CO-0009-647) | 99203 | 22 µl |
Storage
Description
SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
Specificity/Sensitivity
Granzyme B (D6E9W) Rabbit mAb (SignalStar™ Conjugate 0009) recognizes endogenous levels of total Granzyme B protein. Non-specific staining in the sweat glands has been observed. This antibody recognizes human Granzyme B protein and is also reactive with mouse Granzyme B; however, this antibody is not suggested for immunohistochemical analysis of mouse tissues. Instead, Granzyme B (E5V2L) Rabbit mAb #44153 is recommended for IHC analysis of mouse tissue samples. Monkey cross-reactivity is determined by immunohistochemistry.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human Granzyme B protein.
Background
Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
StarBond: SignalStar™ Leica Bond
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStar is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human tonsil using Granzyme B (D6E9W) & CO-0009-647 SignalStar™ Oligo-Antibody Pair #37369 (white), Phospho-SLP-76 (Ser376) (E3G9U) & CO-0018-488 SignalStar™ Oligo-Antibody Pair #34567 (green), CD3ε (D7A6E™) & CO-0001-488 SignalStar™ Oligo-Antibody Pair #92856 (pink), CD20 (E7B7T) & CO-0011-594 SignalStar™ Oligo-Antibody Pair #54189 (yellow), CD8α (D8A8Y) & CO-0004-594 SignalStar™ Oligo-Antibody Pair #19166 (orange), ICOS (D1K2T™) & CO-0027-647 SignalStar™ Oligo-Antibody Pair #24147 (red), Ki-67 (8D5) & CO-0014-750 SignalStar™ Oligo-Antibody Pair #56398 (cyan), Pan-Keratin (C11) & CO-0003-750 SignalStar™ Oligo-Antibody Pair #97227 (magenta), and ProLong Gold Antifade Reagent with DAPI #8961 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
SignalStar™ immunohistochemical analysis of paraffin-embedded human infiltrating ductal carcinoma of the breast using Granzyme B (D6E9W) & CO-0009-488 SignalStar™ Oligo-Antibody Pair #84484 (green). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
SignalStar™ immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Granzyme B (D6E9W) & CO-0009-594 SignalStar™ Oligo-Antibody Pair #15194 (yellow). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
Revision 1
SignalStar™ immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Granzyme B (D6E9W) & CO-0009-647 SignalStar™ Oligo-Antibody Pair #37369 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
SignalStar™ immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using Granzyme B (D6E9W) & CO-0009-750 SignalStar™ Oligo-Antibody Pair #60358 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using Granzyme B (D6E9W) & CO-0009-647 SignalStar™ Oligo-Antibody Pair #37369 (left, red) and ProLong Gold Antifade Reagent with DAPI #8961 (left, blue) compared to chromogenic immunohistochemical analysis of a serial section of paraffin-embedded human squamous cell lung carcinoma using Granzyme B (D6E9W) Rabbit mAb #46890 (right). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
Revision 1
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using Granzyme B (D6E9W) & CO-0009-647 SignalStar™ Oligo-Antibody Pair #37369 (red), LAG3 (D2G4O™) & CO-0026-488 SignalStar™ Oligo-Antibody Pair #69276 (green), CD20 (E7B7T) & CO-0011-594 SignalStar™ Oligo-Antibody Pair #54189 (yellow), HLA-DRA (E9R2Q) & CO-0023-750 SignalStar™ Oligo-Antibody Pair #58446 (cyan), and ProLong Gold Antifade Reagent with DAPI #8961 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human colorectal adenocarcinoma using Granzyme B (D6E9W) & CO-0009-750 SignalStar™ Oligo-Antibody Pair #60358 (cyan) and CD8α (D8A8Y) & CO-0004-594 SignalStar™ Oligo-Antibody Pair #19166 (yellow). All fluorophores have been assigned a pseudocolor, as indicated.
