InTraSeq^™ Single Cell Analysis Resource Center

A) Most single-cell analysis techniques currently available detect RNA and use barcoded antibodies to measure surface proteins for phenotyping purposes. B) Many have tried developing methods to detect intracellular proteins, but these methods typically suffer from significant RNA loss and degradation. C) InTraSeq™ Single Cell Analysis detects RNA alongside intracellular and surface proteins while preserving RNA.

InTraSeq Single Cell Analysis (SCA): An Introduction

Developed and stringently validated by CST scientists, the proprietary formulation of InTraSeq (Intracellular protein and Transcriptomic Sequencing) enables unbiased quantitation of mRNA expression alongside intracellular and cell surface proteins at the single-cell level.

InTraSeq enables researchers to:

• Detect RNA, intracellular, and cell surface proteins in thousands of cells simultaneously while guaranteeing robust RNA signal.
• Investigate intracellular signaling pathways together with the transcriptome at the single-cell resolution.
• Identify hard-to-detect cells with an unbiased depth-of-coverage.
• Explore multiple molecular mechanisms in a single, streamlined experiment.

Below is a collection of resources to inform and support your single-cell analysis research with InTraSeq.

Products

• InTraSeq™ 3’ Assay Kit
• InTraSeq™ Single Cell Analysis Regeants and Kits

Support & Resources

• Custom Conjugation & Labeling Services
• Product Performance Guarantee
• Technical Support