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Render Timestamp: 2024-12-20T10:42:59.204Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-20 06:18:48.017
Product last modified at: 2024-09-20T07:09:56.801Z
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PDP - Template Name: Monoclonal Antibody (Alexa Fluor Conjugate)
PDP - Template ID: *******c8ce56b
  1. Products /
  2. Antibody Conjugates /
  3. Nestin (Rat-401) Mouse mAb (Alexa Fluor® 555 Conjugate)

Nestin (Rat-401) Mouse mAb (Alexa Fluor® 555 Conjugate) #8181

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Inquiry Info. # 8181

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    Supporting Data

    REACTIVITY R
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • R-Rat 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct immunofluorescent analysis in rat brain.

    Product Usage Information

    Application Dilution
    Immunofluorescence (Frozen) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    Nestin (Rat-401) Mouse mAb (Alexa Fluor® 555 Conjugate) recognizes endogenous levels of rat nestin protein.

    Species Reactivity:

    Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with E15 rat spinal cord.

    Background

    Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly downregulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, and astrocytomas, and it is generally accepted as a marker for neural cancer stem cells (3). However, nestin expression has also been observed in astrocytes, retina, cardiac muscle, pancreas, and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.

    Database Links

    UniProt ID: P21263


    Entrez-Gene Id: 25491


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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