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Product Name

4448 AFP (D12C1) Rabbit mAb

Filter:

WB
IP

Inquiry Info. # 2137

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Supporting Data

REACTIVITY	H M
SENSITIVITY	Recombinant
MW (kDa)	70
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

AFP Antibody detects recombinant human and mouse AFP protein. The antibody can only immunoprecipitate human AFP.

Species Reactivity:

Human, Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human AFP. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma (3).

Database Links

UniProt ID: P02771

Entrez-Gene Id: 174

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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AFP Antibody #2137