Render Target: STATIC
Render Timestamp: 2024-12-26T11:27:54.768Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:54:09.404
Product last modified at: 2024-12-17T18:48:50.901Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ARFGAP1 (D9A4V) Rabbit mAb #14522

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 45
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ARFGAP1 (D9A4V) Rabbit mAb recognizes endogenous levels of total ARFGAP1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr338 of human ARFGAP1 protein.

    Background

    ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1) is a Golgi-localized protein that regulates vesicle formation and membrane trafficking (1). ARFGAP1 initiates cargo selection and COP1 vesicle formation by stimulating GTP hydrolysis of ADP-ribosylation factor ARF1 (2). This GTPase activating protein initiates vesicle transport by coupling vesicle formation with cargo sorting (3). ARFGAP1 plays an active role in the Golgi-to-ER retrograde, intra-Golgi, and trans-Golgi trafficking networks (1). Research studies indicate that ARFGAP1 can act as a GTPase activating protein for LRRK2, a large multifunction protein whose genetic mutations are associated with Parkinson’s disease (4). ARFGAP1 regulates GTPase activity and promotes the kinase activity of LRRK2, which suggests some potential as a promising target for study of LRRK2 mediated neurodegeneration (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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