Render Target: STATIC
Render Timestamp: 2024-12-26T11:18:10.909Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-20 06:18:12.846
Product last modified at: 2024-12-17T18:59:00.017Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ATF-6 (D4Z8V) Rabbit mAb #65880

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 90-100
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ATF-6 (D4Z8V) Rabbit mAb recognizes endogenous levels of total ATF-6 alpha protein. The antibody does not detect ATF-6 beta protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ATF-6 alpha protein.

    Background

    Activating Transcription Factor-6 (ATF-6) is a transmembrane glycoprotein of the endoplasmic reticulum (ER). Upon accumulation of improperly folded proteins in the ER, ATF-6 is cleaved liberating a 50 kDa amino-terminal fragment that translocates to the nucleus (1, 2). There, ATF-6 activates transcription of ER chaperones and components of ER-associated degradation such as GRP78, XBP-1, and CHOP (3-5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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