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Render Timestamp: 2024-12-26T11:18:42.836Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-12-10 19:01:09.454
Product last modified at: 2024-12-17T18:47:37.084Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Dab2 (D7O9T) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Dab2 (D7O9T) Rabbit mAb #12906

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 96
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:1600 - 1:3200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Dab2 (D7O9T) Rabbit mAb recognizes endogenous levels of total Dab2 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly388 of human Dab2 protein.

    Background

    Disabled homolog 2 (Dab2) is a mitogen responsive phosphoprotein that exerts multiple functions through association with numerous proteins. Dab2 modulates signaling pathways through interactions with proteins such as Smads and TGF-β receptors (1,2), axin (3), GRB (4), and Src (5). Dab2 also serves as a cargo-specific adaptor of clathrin-mediated endocytosis via interaction with clathrin (6), AP2 (7), NPXY-containing cargo (8-10), and myosin VI (11,12). In addition, Dab2 regulates cell adhesion by directly binding integrins (13,14). The diverse functions of Dab2 enable it to coordinate cell adhesion, cell motility, membrane trafficking, and signaling. Research studies have shown Dab2 is downregulated in a number of cancers, thereby suggesting a role as a tumor suppressor (15-17). Phosphorylation of Dab2 decreases its endocytotic function (18).
    1. Hocevar, B.A. et al. (2001) EMBO J 20, 2789-801.
    2. Hocevar, B.A. et al. (2005) J Biol Chem 280, 25920-7.
    3. Hocevar, B.A. et al. (2003) EMBO J 22, 3084-94.
    4. Xu, X.X. et al. (1998) Oncogene 16, 1561-9.
    5. Zhou, J. et al. (2003) J Biol Chem 278, 6936-41.
    6. Mishra, S.K. et al. (2002) EMBO J 21, 4915-26.
    7. Morris, S.M. and Cooper, J.A. (2001) Traffic 2, 111-23.
    8. Keyel, P.A. et al. (2006) Mol Biol Cell 17, 4300-17.
    9. Maurer, M.E. and Cooper, J.A. (2006) J Cell Sci 119, 4235-46.
    10. Maurer, M.E. and Cooper, J.A. (2005) J Cell Sci 118, 5345-55.
    11. Morris, S.M. et al. (2002) Traffic 3, 331-41.
    12. Hasson, T. (2003) J Cell Sci 116, 3453-61.
    13. Huang, C.L. et al. (2006) J Cell Sci 119, 4420-30.
    14. Teckchandani, A. et al. (2009) J Cell Biol 186, 99-111.
    15. Mok, S.C. et al. (1998) Oncogene 16, 2381-7.
    16. Anupam, K. et al. (2006) World J Gastroenterol 12, 6041-5.
    17. Bagadi, S.A. et al. (2007) Breast Cancer Res Treat 104, 277-86.
    18. Chetrit, D. et al. (2011) J Biol Chem 286, 5392-403.

    Database Links

    UniProt ID: P98082


    Entrez-Gene Id: 1601


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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