Name: EDC4/Ge-1 Antibody
Price: 302.00 USD
Availability: InStock

Filter:

WB
IF

Supporting Data

REACTIVITY	H Mk
SENSITIVITY	Endogenous
MW (kDa)	170
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunofluorescence (Immunocytochemistry)	1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

EDC4/Ge-1 Antibody detects endogenous levels of total EDC4/Ge-1 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human EDC4/Ge-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Enhancer of mRNA decapping 4 (EDC4) was originally identified as the autoantigen Ge-1 from a Sjögren's syndrome patient later diagnosed with primary biliary cirrhosis (1). EDC4 (also known as HEDLS) was later identified as an essential component of cytoplasmic P-bodies responsible for mRNA decapping and degradation (2). Identified EDC4 protein is found as a pair of isoforms generated by alternative splicing and contains several WD domains and a putative nuclear localization signal. EDC4 co-localizes with other P-body decapping proteins such as DCP1A, DCP2 and GW182 (2,3). Experimental evidence suggests that EDC4 may be involved in miRNA-mediated translation repression (4).

Database Links

UniProt ID: Q6P2E9

Entrez-Gene Id: 23644

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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EDC4/Ge-1 Antibody #2548